Naslov
Splenectomy in rats induced portal and caval hypertension, aortic hypotension, venous thrombosis, peaked P waves and tachycardia. Therapy with pentadecapeptide BPC 157

Autori
Žižek, Helena ; Gojković, Slaven ; Krezić, Ivan ; Malekinušić, Dominik ; Petrović, Andrea ; Barišić, Ivan ; Horvat, Katarina ; Drmić, Domagoj ; Knežević, Mario ; Đuzel, Antonija ; Kolovrat, Marijan ; Seiwerth, Sven ; Sikirić, Predrag

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Gastroenterology (New York, N.Y. 1943), 156 (2019), 6(S) / - , 2019, S-563

Skup
Digestive Disease Week

Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 18.05.2019. - 21.05.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
BPC 157 ; splenectomy ; venous thrombosis ; ECG

Sažetak
Aim. We introduce pentadecapeptide BPC 157 therapy in rats after splenectomy, and the whole syndrome going on at 10 min, 3 h and 24 h. Splenectomy includes ligated lineal vein (LV) and artery, gastricae breve veins and arteries. We counteracted thrombosis in the portal vein (PV), inferior caval vein (ICV), superior mesenteric vein (SMV), lienal vein (LV). We counteracted the severe venous hypertension (PV, ICV) and aortal hypotension (assessed as described Vascul Pharmacol 2018 ; 106:54-66), peaked P waves and tachycardia. Recently, in rats with portal triad obstruction, BPC 157 counteracted portal hypertension and caval hypotension (Gastroenterology 2015, Vol. 148, Issue 4, S-650) ; portal and caval hypertension in rats with suprahepatic occlusion of ICV (Gastroenterology, Vol. 154, Issue 6, Suppl 1, s-532) ; portal hypertension in bile duct ligated rats (Gastroenterology 154, Issue 6, Suppl 1, s-536) ; caval hypertension and aortal hypotension and ICV thrombosis after infrarenal ICV occlusion (Vascul Pharmacol 2018 ; 106:54-66). Likewise, BPC 157 counteracts various lesions in the whole GI-tract and free radical formation (Curr Pharm Des 2018 ; 24:1990- 2001). Methods: Medication (/kg) (BPC 157 (10 μg, 10 ng), or saline (5 ml) (controls)) was applied as an abdominal bath immediately after splenectomy, or at 3h or at 24h. Results: Immediately after splenectomy, and continuously thereafter, splenectomied rats exhibit huge PV and ICV hypertension, mild aortic hypotension (means±SD mmHg) (controls, at 10 min, 65±4 (PV), 46±4 (ICV), 71±3 (abdominal aorta), at 3h, 5±4 (PV), 61±4 (ICV), 70±3 (abdominal aorta), at 24h, 38±4 (PV), 47±4 (ICV), 68±3 (abdominal aorta)) and marked thrombosis (cloth weight, mg), at 10 min 9.5±0.5 ICV, 6.6±0.9 PV, 4.8±0.9 SMV, 1.3±0.6 LV, at 3h 10.1±0.5 ICV, 5.3±0.8 PV, 19.2±0.9 SMV, 1.0±0.6 LV, at 24 h 33.8±2.5 ICV, 27.5±2.9 PV, 8.8±0.9 SMV, 3.8±0.6 LV), unless BPC 157 was given (10 μg: at 10 min 29±4 (PV), 20±4 (ICV), 87±3 (abdominal aorta), at 3h , 20±4 (PV), 17±4 (ICV), 81±3 (abdominal aorta), at 24h, 12±4 (PV), 20±4 (ICV), 82±3 (abdominal aorta)) and (cloth weight, mg, at 10 min 2.9±0.5 ICV, 2.6±0.9 PV, 3.2±0.3 SMV, 0.5±0.2 LV, at 3h 6.3±0.5 ICV, 2.3±0.5 PV, 5.9±0.9 SMV, 0.6±0.2 LV, at 24 h 12.2±2.5 ICV, 1.9±0.5 PV, 4.8±0.9 SMV, 2.0±0.6 LV)), (*P≤0.05, at least vs. control). All control rats after splenectomy exhibit peaked P waves and tachycardia. USB camera recording documented full congestion of the PV, SMV and its tributaries. Contrarily, no congestion of the PV, SMV and its tributaries appears along with BPC 157 administration in all BPC 157-rats and no peaked P waves and tachycardia (Fisher exact probability test P≤0.05 vs. control) (Fig. 1, control left, BPC 157 right). Conclusion. BPC 157 therapy successfully counteracts the adverse effects of the splenectomy, hemodynamic disturbances, peaked P waves, tachycardia.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA