Naslov
Presence and antimicrobial resistance of Staphylococcus aureus in milk from vending machines

Autori
Kiš, Marta ; Kolačko, Ivana ; Zdolec, Nevijo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Archives of industrial hygiene and toxicology / Šostar, Zvonimir ; Šikić, Sandra ; Krivohlavek, Adela ; Bošnir, Jasna - Zagreb, 2020

Skup
3rd International Congress on Food Safety and Quality: Food, Health and Climate Changes

Mjesto i datum
Zagreb, Hrvatska, 10.11.2020. - 13.11.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
disk diffusion method ; isolates ; microbiological safety ; milk distribution chain ; raw milk

Sažetak
Consumption of milk from vending machines is popular in Croatia, but it is necessary to evaluate the potential risks to consumers in terms of microbiological safety. The aim of this study was to examine the presence and counts of Staphylococcus aureus in raw milk from vending machines (n=319) and to test the susceptibility of isolates to antimicrobials over a one-year period. The number of S. aureus was determined according to HRN EN ISO 6888 and the assessment of resistance to kanamycin, erythromycin, nitrofurantoin, ampicillin, levofloxacin, teicoplanin, ciprofloxacin, penicillin, cefotaxime, tetracycline, trimethoprim/sulfamethoxidolol, linezolid, chloramphenicol, and ceftazidim by disk diffusion method. The average number of S. aureus by months of sampling ranged from 2.04 to 3.09 log CFU/mL. A total of 88 samples contained these bacteria (27.58 %). Sensitivity to antimicrobials was tested in 60 isolates and the percentage of resistant isolates varied from 33.3 to 100 % depending on the sampling month. A total of 41 isolates were resistant to at least one antimicrobial agent (68.33 %). The majority of isolates were resistant to penicillin, ampicillin, and ciprofloxacin, followed by linezolid, ceftazidime, kanamycin, and levofloxacin. Based on our results, there is a strong need to implement appropriate control measures in order to reduce microbiological risk and within connected antimicrobial resistance in the milk distribution chain through vending machines.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA