Naslov
Establishment of Reference Intervals for Automated Light Transmission Aggregometry

Autori
Coen Herak, Desiree ; Dolčić, Maja ; Herceg Brlek, Tihana ; Miloš, Marija ; Lapić, Ivana ; Zadro, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Research and Practice in Thrombosis and Haemostasis / Cushman, Mary - : John Wiley & Sons, 2020, 333-334

Skup
Annual Meeting of the International Society on Thrombosis and Haemostasis (ISTH 2020)

Mjesto i datum
Online, 12.07.2020. - 14.07.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Reference intervals ; aggregometry ; automation

Sažetak
Background: Transition from time consuming and labor intensive manual to automated light transmission aggregometry (LTA) requires the establishment of agonist specic reference intervals. Aims: The aim of the study was to establish reference intervals for the standard panel of agonists on the coagulation analyzer Sysmex CS-2500 (Siemens Healthineers, Germany). Methods: At least 20 healthy volunteers with normal platelet values were included in the study. Maximal aggregation response was measured in platelet rich plasma samples according to manufacturer instructions (acquisition time of 600 seconds), using the following agonists and nal concentrations: adenosin diphosphate (ADP, 2 and 5 µM), arachidonic acid (AA, 1 mM), collagen (2 and 4 mg/L), epinephrine (5 and 10 µM) and ristocetin (1.2 g/L). All agonist were from HYPHEN Biomed (Neuville sur Oise, France). For each agonist concentration duplicate measurements were performed and the mean maximal aggregation (%) was used for the establishment of reference intervals, by nding the 2.5 to 97.5 percentile for each agonist and agonist concentration. The MedCalc program version 9.3.2.0 for Windows (MedCalc Software, Mariakerke, Belgium) was used for statistical analysis. Results: The following reference intervals were obtained: 69.8-92.4% for AA (N=21), 63.4-90.9% for 2 µM ADP (N=26) and 67.1-93.0% for 5 µM ADP (N=26), 62.3-94.1% for 2 mg/L collagen (N=26) and 70.9- 93.4% for 4 mg/L collagen (N=21), 63.1-92.1% for 5 µM epinephrine (N=22) and 63.4-95.6% for 10 µM epinephrine (N=21), while the obtained reference interval for ristocetin (N=20) was 71.3-91.7%. Conclusions: Reagent specic reference intervals for automated LTA on Sysmex CS-2500 were established for the standard agonist panel and introduced into routine practice for our patient population.

Izvorni jezik
Engleski

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