Naslov
Sequence conservation of the IDL regions and the acidic TIPs within eubacterial SSB proteins

Autori
Paradžik, Tina ; Filić, Želimira ; Vujaklija, Dušica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Book of abstracts of Power of microbes in industry and environment 2019 / - , 2019, 61-61

Skup
Power of Microbes 2019

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 15.05.2019. - 18.05.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
SSB ; IDL regions ; sequence ; bacteria

Sažetak
SB (single-stranded DNA binding) proteins are essential in all domains of life. These proteins bind and protect single-stranded DNA (ssDNA) during DNA recombination, replication and repair. With some exceptions, the eubacterial SSB proteins act as a homotetramers. Each monomer has two distinct domains: N-terminal domain with a conserved oligonucleotide-oligosaccharide fold (OB-fold) responsible for ssDNA binding, and C-terminal domain (Ct) with conserved acidic tip (TIP). Ct is intrinsically disordered (IDL) and play important role in mediating SSB-protein interactions. Recently, rrepetitive motifs, PXXP and GGX, which contibutes to elastomeric properties were found within IDL regions of SSB proteins. The aims of this study were: (i) To analyse the conservation of PXXP and GGX elements in IDL regions with respect to GC content of bacterial SSBs as well as the conservation of the acidic TIPs, (ii) to examine the impact of increased glycine/proline ratio in IDLs on the variations of these motifs in actinobacterial SSBs. SSB sequences were collected from genomes covering a wide range of GC content from 14 bacterial phyla. The ML trees were constructed for SSB sequences and corresponding 16S rRNA genes. The amino acids (aa) content of OB folds and IDL regions and TIPs were subsequently analysed. Variability and the presence of PXXP and GGX motives within IDLs were inspected. SSB protein sequence analyses suggested a correlation between amino aa composition and GC content. We will show how two distinct domains of SSB exhibit significant difference to the expected aa composition. In line with this, we will discuss the conservation of PXXP and GGX motives in SSBs within analysed bacterial phyla and with the respect to GC content.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA