Pregled bibliografske jedinice broj: 1084542
Chronic neurodegeneration and glial response following repetitive mild traumatic brain injury in the mouse optic tract are not affected by TDP-43 proteinopathy
Chronic neurodegeneration and glial response following repetitive mild traumatic brain injury in the mouse optic tract are not affected by TDP-43 proteinopathy // FENS 2020 Virtual Forum of Neuroscience
Glasgow, Ujedinjeno Kraljevstvo, 2020. str. - (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1084542 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Chronic neurodegeneration and glial response following repetitive mild traumatic brain injury in the mouse optic tract are not affected by TDP-43 proteinopathy
Autori
Dolenec Petra, Pilipović Kristina, Rajič Bumber Jelena, Janković Tamara, Gržeta Nika, Križ Jasna, Župan Gordana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
FENS 2020 Virtual Forum of Neuroscience
/ - , 2020
Skup
FENS 2020 Virtual Forum of Neuroscience
Mjesto i datum
Glasgow, Ujedinjeno Kraljevstvo, 11.07.2020. - 15.07.2020
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
repetitive traumatic brain injury, optic tract, neurodegeneration, glia, mouse
Sažetak
Aims: Preclinical and clinical studies suggest visual system vulnerability to damage following repetitive mild traumatic brain injury (rmTBI). Increasing evidence points to association between rmTBI and neurodegenerative diseases in which Tar DNA binding protein 43 (TDP-43) neuropathology was observed. The aim of this study was to investigate if rmTBI induces chronic neurodegeneration and glial response in the optic tracts of mice and does the genetic predisposition to TDP-43 proteinopathy influence it. Methods: Brain traumas were induced by the weight drop method, during five consecutive days, twice daily in wild type C57BL/6J (WT) and transgenic TDP-43G348C (TGTDP-43) adult mice. Mice of the control groups were anesthetized without receiving head impacts. Animals were sacrificed 6 months after the last procedure and their brains were prepared for the histological analyses. Fluoro-Jade C staining was used for detection of neurodegeneration, and immunofluorescent labeling of Iba1 and GFAP for microglia and astrocytes identification. Results: We found significantly increased Fluoro-Jade C intensity levels as well as the Iba1 and GFAP immunoreactivities in the optic tracts of traumatized mice of both genotypes in comparison to the related control groups, but the differences between the injured WT and TGTDP-43 animals were not detected. Conclusions: Our research suggests the presence of chronic degeneration and pronounced glial activity in the mouse optic tracts 6 months following rmTBI that was not additionally affected by genetically acquired TDP-43 dysregulation. This research was fully supported by the Croatian Science Foundation under project IP-2016-06-4602 to Ž.G.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-4602 - Blaga repetitivna traumatska ozljeda mozga: model za istraživanje neuropatologije i neuroupale posredovane s TDP-43 (TDP-43repTBI) (Župan, Gordana, HRZZ - 2016-06) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Petra Dolenec
(autor)
Jelena Rajič Bumber
(autor)
Tamara Janković
(autor)
Gordana Župan
(autor)
Jasna Križ
(autor)
Nika Gržeta
(autor)
Kristina Pilipović
(autor)