Pregled bibliografske jedinice broj: 1083738
Characterization of a lipid-producing thermotolerant marine photosynthetic pico-alga in the genus Picochlorum (Trebouxiophyceae)
Characterization of a lipid-producing thermotolerant marine photosynthetic pico-alga in the genus Picochlorum (Trebouxiophyceae) // European journal of phycology, 55 (2020), 4; 384-399 doi:10.1080/09670262.2020.1757763 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1083738 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Characterization of a lipid-producing
thermotolerant marine photosynthetic pico-alga
in the genus Picochlorum (Trebouxiophyceae)
Autori
Mucko, Maja ; Padisák, Judit ; Gligora Udovič, Marija ; Pálmai, Tamás ; Novak, Tihana ; Medić, Nikola ; Gašparović, Blaženka ; Peharec Štefanić, Petra ; Orlić, Sandi ; Ljubešić, Zrinka
Izvornik
European journal of phycology (1469-4433) 55
(2020), 4;
384-399
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Morphology ; Picochlorum ; photosynthetic picoeukaryotes ; phylogeny ; physiology
Sažetak
A new marine strain of picoplanktonic algae, PMPFPPE4, was isolated from a mixed net- phytoplankton sample taken from the upper euphotic layer of the southeastern Adriatic Sea. Evaluation of the new strain included morphological investigation (by light and electron microscopy), phylogenetic analysis (utilizing plastid 16S rRNA and nuclear 18S rRNA genes), and physiological characterization (screening of pigment/lipid composition and capturing photosynthesis measurements). The new strain was proven to belong to the genus Picochlorum and the lipid composition revealed an unexpected accumulation of triacylglycerols, indicating an evolutionary adaptation for growth under unfavourable conditions. In addition, lipid remodelling in the exponential to stationary growth phase was characterized by an increased share of membrane-forming digalactosyldiacylglycerols and phosphatidylcholines. Maximum photosynthetic activity measured was at 30°C, but the most rapid increase of photosynthetic activity was at lower temperatures (15–20°C). Moreover, the thermotolerant strain did not exhibit photoinhibition below 40°C and survived a one- month cultivation period in complete darkness. The strain’s survival in low light and dark conditions suggests a potential shift from autotrophy to mixotrophy under unfavourable growth conditions. Thus, the unique physiological attributes represented by a high growth rate, thermotolerance, phototolerance and high triacylglycerol synthesis may render the strain highly attractive for biofuel production and growth in large outdoor systems.
Izvorni jezik
Engleski
Znanstvena područja
Interdisciplinarne prirodne znanosti
POVEZANOST RADA
Projekti:
HRZZ-IP-2014-09-2945 - Utjecaj promjene termohaline cirkulacije u istočnom Mediteranu na planktonske zajednice u južnom Jadranu: ekološki i genetički pristup (AdMedPlan) (Batistić, Mirna, HRZZ ) ( CroRIS)
HRZZ-UIP-2013-11-6433 - Bioindikatori vodenih masa u Jadranu (BIOTA) (Ljubešić, Zrinka, HRZZ - 2013-11) ( CroRIS)
IP-2013-11-8607 - Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu (AMBIOMERES) (Gašparović, Blaženka, HRZZ - 2013-11) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb
Profili:
Sandi Orlić
(autor)
Marija Gligora Udovič
(autor)
Maja Mucko
(autor)
Zrinka Ljubešić
(autor)
Petra Peharec Štefanić
(autor)
Blaženka Gašparović
(autor)
Tihana Novak
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI