Naslov
Karakterizacija novonastalog tkiva nakon biopsije hrskavice nosne pregrade
(Characterization of newly-formed tissue after nasal septum cartilage biopsy)

Autori
Brezak, Matea

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
12.02

Godina
2019

Stranica
29

Mentor
Marijanović, Inga

Neposredni voditelj
Pušić, Maja

Ključne riječi
ekspresija gena, hrskavica nosnog septuma, imunohistokemijska analiza, perihondrij, tkivno inženjerstvo
(gene expression, immunohistochemical analysis, nasal cartilage, perichondrium, tissue engineering)

Sažetak
Tissue engineering has become one of the most studied fields with clinical application. When producing cartilage tissue in vitro, nasal septum biopsy is taken in order to harvest chondrocytes that will be seeded on scaffolds. Following this procedure, the nasal septum is left with a perforation that fills in over time. The newly- formed tissue may not possess functional properties characteristic for septum cartilage, whereas if the neocartilage forms it would enable chondrocyte reharvesting and confirm normal septum function. To this time, no one has addressed the issue of tissue formation in the place of the perforation on a large animal model. Therefore, in this study, newly-formed tissue was characterized using histological and immunohistochemical stainings including analysis of cartilage and perichondrium marker gene expression. Low regenerative potential of cartilage was confirmed as no formation of similar tissue was observed over time. Perichondral origin of newly-formed tissue was confirmed both histologically and through gene expression analysis. Additionally, high expression of COL 1, COL 4 and CRABP 1 genes indicate a significant role of outer perichondrium layer in tissue genesis.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA