Naslov
Iron oxide nanoparticles as a tool for MRI tracking of nasal chondrocytes

Autori
Pusic, Maja ; Marijanovic, Inga ; Skokic, Sinisa ; Horak, Daniel ; Gajovic, Srecko ; Vuckovic, Mirta ; Kostesic, Petar ; Maticic, Drazen ; Vnuk, Drazen ; Ivkovic, Alan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts- 5th TERMIS World Congress / - , 2018, 636-636

Skup
5th TERMIS World Congress

Mjesto i datum
Kyoto, Japan, 04.09.2018. - 07.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
iron oxide nanoparticles, cartilage, nasal chondrocytes, MRI tracking

Sažetak
Superparamagnetic iron oxide nanoparticles (SPION) can be used as contrast agents for tracking and visualization of cells and tissues with magnetic resonance imaging (MRI). In this study, we focused on two types of nanoparticles, D-mannose coated and PEG coated iron oxide nanoparticles. These nanoparticles showed low cytotoxicity properties and were detectable with MRI in previous studies. Since nasal chondrocytes (NC) are used in clinical study for treatment of articular cartilage defects, we wanted to examine the possibility of NC labelling with iron oxide nanoparticles and to assess their effect on chondrocyte viability and differentiation. Sheep nasal septum cartilage biopsies were dissected and digested in collagenase solution to obtain NC. Isolated chondrocytes were expanded in expansion medium until passage 2 and incubated with different concentrations of D-mannose γ- Fe2O3 and Fe3O4&SiO2-PEG nanoparticles for 1.5, 24, 48 and 72 h. Nanoparticle cytotoxicity was determined by Crystal violet staining method and Live Dead assay. In order to test chondrocytes differentiation potential in the presence of PEG and D-mannose coated nanoparticles, three dimensional pellet culture was established. Pellets were collected after 1, 7 and 14 days. For histological evaluation, pellets were stained with Safranin O and Prussian blue, and immunostained against collagen type II. Expression of collagen type I, II and aggrecan was determined by qPCR. To confirm detection of nanoparticles with MRI, T2 and T2* weighted sequences were obtained. Viability assays showed no significant reduction in chondrocyte viability after the treatment with D-mannose-coated and PEG-coated nanoparticles. Chondrocytes treated with D-mannose nanoparticles had higher proliferation rate than control cells or cells treated with PEGcoated nanoparticles. Histological staining and qPCR for chondrogenic markers confirmed secreted extracellular matrix in treated chondrocytes as in control cells. Prussian blue staining and MRI T2 and T2* maps confirmed presence of D-manose nanoparticles in pellets after 7 and 14 days. D-mannose coated iron oxide nanoparticles do not reduce viability and proliferation of NC. Their redifferentiation capacity also remained unaffected. Labeling efficiency of NC with D-mannose nanoparticles make them a potential agent for chondrocyte tracking after graft implantation.

Izvorni jezik
Engleski

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