Naslov
Izolacija i strukturna karakterizacija komponenata reakcijskih smjesa 9a- i 4''- tetrahidrofurfuril makrozona tehnikom LC- SPE/NMR
(Isolation and structural characterization of 9a- and 4''-tetrahydrofurfuryl macrozone reaction mixture components using the LC- SPE/NMR)

Autori
Sekula, Lucia Ema ; Habinovec, Iva ; Mikulandra, Ivana ; Gašperov, Jana ; Novak, Predrag ; Grgičević, Ivan ; Bukvić, Mirjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Adriatic NMR conference 2020 : Book of abstracts / Bregović, Nikola ; Namjesnik, Danijel ; Novak, Predrag ; Parlov-Vuković, Jelena - Zagreb, 2020, 38-38

ISBN
978-953-6076-58-1

Skup
4th Adriatic NMR conference 2020

Mjesto i datum
Peroj, Hrvatska; Vodnjan, Hrvatska, 22.09.2020. - 24.09.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
tehnika LC-SPE-NMR, analiza onečišćenja, makrozoni
(LC-SPE-NMR technique, impurity profiling, macrozones)

Sažetak
Due to a global problem of growing bacterial resistance, discovery of new antibiotics is of an outmost importance and various studies are in progress to overcome the resistance mechanisms.[1- 3] Macrozones are novel bioactive conjugates of azithromycin and thiosemicarbazides that posses very good in vitro antibacterial activity against selected Gram-positive and Gram-negative bacteria. [4] Efficient and rapid purification and isolation of newly synthesized bioactive compounds is a crucial step prior to biological evaluation. Furthermore, isolation and identification of impurities is very important procedure during the drug development in pharmaceutical industry. Preparative and semipreparative HPLC techniques are classical purification methods, but they can be time and solvent consuming. In this study we have successfully used hyphenated LC-SPE/NMR approach for the isolation and structural characterization of main components and some impurities in 9a- and 4''- tetrahydrofurfuryl macrozone reaction mixtures. Structures of the isolated compounds were proposed on the basis of the analysed one- and two-dimensional NMR spectra and MS spectra, respectively.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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