Naslov
Cytokines produced by human B cells enable them to maintain rather than establish T helper cell responses

Autori
Gagro, Alenka ; Toellner, K M ; Servis, Dražen ; Grafton, G ; Gordon, John

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Scandinavian Journal of Immunology 54, (Suppl 1) / - , 2001, 32-32

Skup
11th International Congress of Immunology

Mjesto i datum
Stockholm, Švedska, 22.07.2001. - 27.07.2001

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Sažetak
B cells, in their resting state, lack antigen presentation capacity, but shortly after crosslinking of their Ig and/or ligation of CD40, they upregulate B7 molecules and differentiate into efficient antigen presenting cells (APC). The recent finding that murine naive B cells differentiate into effector subsets 1 and 2, which produce Th1- and Th2-associated cytokines, respectively, has suggested that B cells have the capacity to amplify or maintain an ongoing polarized immune responses. We have shown previously that exogenous IL-12 induced IFN-gamma production in human B cells on engaging CD40.Here we compared human naive (CD27-ve) and memory (CD27+ve cells) tonsilar B cells for their expression of the major Th1 polarising cytokine, IL-12, and signals that induce this cytokine. We found that, in contrast to dendritic cells, CD40 ligation in the presence of IFN-gamma, did not induce IL-12 secretion in either naive or memory B cells (determined by ELISA and intracellular cytokine staining) although both freshly isolated and in vitro stimulated populations expressed mRNA for p40 IL-12. The inability of B cells to produce IL-12 would limit their capacity as first line APC for the initiation of Th1 responses. However, their capacity to produce IFN-gamma within a Th1-priming environment offers B cells the potential to maintain and amplify this direction of skewing

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA