Naslov
Cytokines produced by human B cells enable them to maintain rather than establish T helper cell responses

Autori
Gagro, Alenka ; Toellner, K.M. ; Grafton, G. ; Gordon, John

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book / - , 2001, 49-49

Skup
Keystone Symposium "B Lymphocyte Immunobiology and Disease"

Mjesto i datum
Snowbird (UT), Sjedinjene Američke Države, 16.04.2001. - 22.04.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Sažetak
The recent finding that murine na&iuml ; ve B cells can differentiate into effector subsets 1 and 2, which produce Th1- and Th2-polarising cytokines, respectively, has suggested that B cells have the capacity to amplify or maintain an ongoing polarised immune response. The activation requirements of naive vs memory B cells for cytokine production could be different. Here we compared human na&iuml ; ve (IgM+IgD+CD27-CD38-IgG-IgA-) and memory (IgM-IgD-CD27+CD38-IgG+/-IgA+/-) tonsillar B cells for their expression of IL-12 and IL-23. We found that B cell subsets when engaged in signaling via BCR and CD40 do not modulate p35 IL-12 as measured by quantitative PCR. CD40 ligation induced p40 IL-12 measured both by RT-PCR and p40 ELISA significantly more in memory B cells. Neither B cell subsets secreted significant amounts of p70 IL-12. Memory B cells also expressed more p19 IL-23 mRNA following BCR ligation. The inability of B cells to produce the p70 IL-12 heterodimer would limit their capacity as frontline APC for the initiation of Th1 responses. However, the ability of memory B cells to produce IL-23 offers these cells the capacity to maintain and amplify Th1-direction of skewing following subsequent encounters with the antigen

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA