Pregled bibliografske jedinice broj: 1080795
The possibility for development of non-transgenic rat tauopathy model by application of tau oligomers into the entorhinal cortex
The possibility for development of non-transgenic rat tauopathy model by application of tau oligomers into the entorhinal cortex // 9th Croatian Congress of Pharmacology with International Participation Book of Abstracts
Zagreb, Hrvatska, 2019. str. 123-123 (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 1080795 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The possibility for development of non-transgenic rat tauopathy model by application of tau oligomers into the entorhinal cortex
Autori
Langer Horvat, Lea ; Španić, Ena ; Šimić, Goran
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
9th Croatian Congress of Pharmacology with International Participation Book of Abstracts
/ - , 2019, 123-123
Skup
9. hrvatski kongres farmakologije = 9th Croatian Congress of Pharmacology
Mjesto i datum
Zagreb, Hrvatska, 25.09.2019. - 28.09.2019
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
Alzheimer's disease ; animal model ; cognitive impairment ; entorhinal cortex ; tauopathy ; tau proteins
Sažetak
Introduction: Alzheimer's disease (AD) is the most common secondary tauopathy characterized by progressive loss of cognitive functions and behavioral impairment. Materials and Methods: Four-month-old male Wistar rats were stereotaxically injected into the entorhinal cortex with pathological tau oligomers, tau fibrils, and PBS (phosphate-buffered saline). Animals were tested, sacrificed, and analyzed 4, 8, and 11 months post-injection. Cognitive performance testing included T-maze, novel object recognition test, and object location test. To detect specific tau protein changes and perform staging of tau pathology, we used several anti-tau monoclonal antibodies. Proteins isolated from the entorhinal cortex and hippocampus were analyzed by immunoblotting. Results: The obtained results suggested that the stereotaxic injection of pathological tau oligomers or tau fibrils into the lateral entorhinal cortex induced phosphorylation of Ser202/Thr205 tau epitope. Using the HT7 antibody, which recognizes human tau protein, we also found a signal present in the brainstem and transentorhinal region. Rewarded learning in the T-maze showed learning curve with more incorrect choices in rats injected with tau fibrils. Conclusion: Understanding of the role of tau oligomers and tau fibrils in this rat model of neurodegeneration has a great potential for revealing mechanisms underlying development and progression of AD in humans.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti, Psihologija, Kognitivna znanost (prirodne, tehničke, biomedicina i zdravstvo, društvene i humanističke znanosti)
