Pregled bibliografske jedinice broj: 1076414
Stratum corneum biomarkers in atopic dermatitis: biological and spatial variability
Stratum corneum biomarkers in atopic dermatitis: biological and spatial variability // The Open biomarkers journal, 10 (2020), 47-54 doi:10.2174/1875318302010010047 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1076414 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Stratum corneum biomarkers in atopic dermatitis:
biological and spatial variability
Autori
Jurakić Tončić, Ružica ; Kežić, Sanja ; Ljubojević Hadžavdić, Suzana ; Marinović, Branka ; Jakaša, Ivone
Izvornik
The Open biomarkers journal (1875-3183) 10
(2020);
47-54
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
atopic dermatitis ; biomarkers ; non-invasive ; cytokines ; filaggrin ; stratum corneum
Sažetak
Background: Atopic dermatitis is a highly heterogeneous skin disease, mainly affecting children. Introduction of biological therapies has urged the development of biomarkers to facilitate personalized therapy. Stratum corneum biomarkers emerged as a promising non-invasive alternative to skin biopsy, yet validation of spatial and biological variability is essential for their application in clinical research. Objective: To assess spatial and biological variability of stratum corneum biomarkers for atopic dermatitis. Methods: Stratum corneum was collected from 17 atopic dermatitis patients by consecutive application of eight adhesive tapes to a lesional skin site and 2 cm and 4 cm from the lesion. Two non-lesional sites at a 2 cm distance from the same lesion were collected to determine biological variability. Filaggrin degradation products (NMF) were determined by liquid chromatography and thirteen cytokines (IL-4, IL-13, IL-18, IL-31, IL- 33, CCL17, CCL22, CCL27, CXCL8, IL-1α, IL-RA, IL- 18, IL-22) by multiplex immunoassay. Results: Biomarker levels showed gradual changes from lesional to non-lesional skin sites at 2 cm and 4 cm ; magnitude and direction of change were biomarker-specific. Intra-subject variability ranged from 17.3% (NMF) to 85.1% (CXCL8). Biomarker levels from two stratum corneum depths were highly correlated ; several biomarkers showed significant depth dependence. Conclusion: Stratum corneum enables non-invasive collection of relevant immune and epidermal biomarkers, but biomarker-specific spatial and biological variability emphasizes the importance of standardized procedures for stratum corneum collection.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
IP-2018-01-5978 - Klinički biomarkeri atopijskog dermatitisa (ADBIOMARK) (Marinović, Branka, HRZZ - 2018-01) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb,
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb
Profili:
Ružica Jurakić Tončić
(autor)
Suzana Ljubojević Hadžavdić
(autor)
Sanja Kežić
(autor)
Ivone Jakasa
(autor)
Branka Marinović
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Scopus