Naslov
UPLC-MS/MS Characterization of Phenolic Compounds and Antioxidant Capacity of Pistacia Lentiscus L. Leaf Extract Isolated Using Microwave-Assisted Extraction

Autori
Kruk, Valentina ; Repajić, Maja ; Elez Garofulić, Ivona ; Zorić, Zoran ; Pedisić, Sandra ; Dragović-Uzelac, Verica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SHIFT20 Virtual Poster Sessions / - , 2020

Skup
SHIFT20 - IFT Virtual Annual Meeting 2020

Mjesto i datum
Chicago (IL), Sjedinjene Američke Države, 13.07.2020. - 15.07.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mastic tree ; UPLC-MS/MS ; phenolic compounds ; antioxidant ; microwave-assisted extraction

Sažetak
Introduction: Pistacia lentiscus L. as wild-growing and widespread annual dioecious evergreen shrub possess a great economic potential due to its widespread application in food, pharmaceutical and other industries. It has been used in traditional medicine since ancient times due to the content of various biologically active molecules, such as polyphenols which offer many pharmacological effects. Hence, Pistacia lentiscus L. leaf can be used for the production of plant extracts using novel extraction technique, such as microwave-assisted extraction. Method: In this study, the aim was to examine the influence of microwave-assisted extraction on the yield of total phenols from Pistacia lentiscus L. leaf. Temperature (50 °C –70 °C), power (200-500 W) and time (4-12 min) were varied. Phenolic profile of Pistacia lentiscus L. leaf extract was determined using UPLC-MS/MS. Antioxidant capacity was determined by ORAC method. The experimental design and statistical analysis were carried out using Statsoft STATISTICA v. 12. The Central Composite Design was used to determine the influence of temperature, microwave power and extraction time on concentration of phenolic compounds in obtained extracts. The validity of the quadratic empirical model was tested using the analysis of variance (ANOVA) with the confidence level 95%. Results: With temperature and extraction time increase, the concentration of phenolic compounds increased and the established conditions for optimal extraction were: temperature 69 °C, time 12 minutes and microwave power 512 W. Using these conditions, obtained total phenols concentration was 108.14 mg GAE/g sample. Identification and quantification of total phenols have been implemented with UPLC-MS/MS and 34 phenolic compounds were identified. Derivates of gallic acid, myricetin and quercetin were defined at the highest concentration. High antioxidant activity of the Pistacia lentiscus L. leaf extract (10768.2±0.07 μmol TE/L) was determined by ORAC method. Significance: Based on conducted research and collected results microwave-assisted extraction has been proven effective for the production of valuable extracts from Pistacia lentiscus L. leaf which has great potential for use in the pharmaceutical industry and food industry as a good base for producing functional products. The advantages of microwave-assisted extraction are higher efficiency, shorter extraction time, less solvent consumption as well as the possibility of using „green solvents”.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

Napomena
Ovo je istraživanje provedeno je u sklopu projekta "Bioaktivne molekule ljekovitog bilja kao prirodni antioksidansi, mikrobiocidi i konzervansi" (KK.01.1.1.04.0093)sufinanciranim sredstvima vlade Republike Hrvatske i
Europske unije putem Europskog fonda za regionalni razvoj-Operativni program Konkurentnost i kohezija 2014. -2020.

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