Naslov
Quantitative structural organization of bulk apical membrane traffic in pollen tubes

Autori
Grebnev, Gleb ; Cvitković, Mislav ; Fritz, Carolin ; Cai, Giampiero ; Smith, Ana-Sunčana ; Kost, Benedikt

Izvornik
Plant physiology (0032-0889) 183 (2020), 4; 1559-1585

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
structural organization ; membrane traffic ; polen tubes

Sažetak
Pollen tube tip growth depends on balancing secretion of cell wall material with endocytic recycling of excess material incorporated into the plasma membrane (PM). The classical model of tip growth, which predicts bulk secretion occurs apically and is compensated by subapical endocytosis, has been challenged in recent years. Many signaling proteins and lipids with important functions in the regulation of membrane traffic underlying tip growth associate with distinct regions of the pollen tube PM, and understanding the mechanisms responsible for the targeting of these regulatory factors to specific PM domains requires quantitative information concerning the sites of bulk secretion and endocytosis. Here, we quantitatively characterized the spatial organization of membrane traffic during tip growth by analyzing steady-state distributions and dynamics of FM4-64-labeled lipids and YFP-tagged transmembrane (TM) proteins in tobacco (Nicotiana tabacum) pollen tubes growing normally or treated with Brefeldin A to block secretion. We established that 1) secretion delivers TM proteins and recycled membrane lipids to the same apical PM domain, and 2) FM4-64-labeled lipids, but not the analyzed TM proteins, undergo endocytic recycling within a clearly defined subapical region. We mathematically modelled the steady- state PM distributions of all analyzed markers to better understand differences between them and to support the experimental data. Finally, we mapped subapical F-actin fringe and trans- Golgi network positioning relative to sites of bulk secretion and endocytosis to further characterize functions of these structures in apical membrane traffic. Our results support and further define the classical model of apical membrane traffic at the tip of elongating pollen tubes.

Izvorni jezik
Engleski

Znanstvena područja
Fizika

Napomena
FUNDING
Deutsche Forschungsgemeinschaft (DFG)
RTG 1962 P7
Gleb Grebnev, Benedikt Kost
Deutsche Forschungsgemeinschaft (DFG)
RTG 1962 P10
Mislav Cvitkovic, Ana-Suncana Smith
EC | European Research Council (ERC)
2013-33728
Mislav Cvitkovic, Ana-Suncana Smith
Deutsche Forschungsgemeinschaft (DFG)
INST90/1074-1FUGG
Benedikt Kost
Deutsche Forschungsgemeinschaft (DFG)
INST90/1025-1FUGG
Benedikt Kost

POVEZANOST RADA