Pregled bibliografske jedinice broj: 1071215
Serum proteome profiling in canine chronic valve disease using a TMT-based quantitative proteomics approach
Serum proteome profiling in canine chronic valve disease using a TMT-based quantitative proteomics approach // Journal of Proteomics, 223 (2020), 103825, 9 doi:10.1016/j.jprot.2020.103825 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1071215 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Serum proteome profiling in canine chronic
valve disease using a TMT-based quantitative
proteomics approach
Autori
Kuleš, Josipa ; Bilić, Petra ; Horvatić, Anita ; Kovačević, Alan ; Guillemin, Nicolas ; Ljubić, Blanka Beer ; Galan, Asier ; Jović, Ines ; Torti, Marin ; Rubić, Ivana ; Eckersall, Peter David ; Mrljak, Vladimir
Izvornik
Journal of Proteomics (1874-3919) 223
(2020);
103825, 9
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Chronic valve disease ; Dog ; Serum ; TMT-based quantitative proteomics ; Biomarker
Sažetak
Chronic valve disease (CVD) is the most common clinically significant heart disease of dogs, affecting 20 to 40% of dogs. The aim of this study was to evaluate the serum protein profile of healthy and CVD affected dogs, by means of an isobaric tandem mass tag (TMT) label-based high-resolution quantitative proteomic approach. Additionally, conventional cardiac biomarkers were measured in the serum, functional bioinformatics analysis was employed for elucidating molecular mechanisms and pathways associated with CVD, and validation of proteomic results was performed by immunoassays and Western blotting. Of 290 identified and quantified proteins, 15 proteins showed significantly different abundances (p < .05), including antithrombin-III, alpha-2- antiplasmin, tetranectin, apolipoprotein M, adiponectin, inter-alpha-trypsin inhibitor heavy chain H1, gelsolin and apolipoprotein B- 100. The identified proteins with differently abundances are involved in a number of pathways, such as complement and coagulation cascades, haemostasis, regulation of actin cytoskeleton, lipid metabolism and transport. We found comparative similarities with human disease in terms of identified proteins and GO pathways, which confirmed similar pathophysiology of this disease, but also differences, mainly in lipid metabolism.
Izvorni jezik
Engleski
Znanstvena područja
Veterinarska medicina
POVEZANOST RADA
Ustanove:
Veterinarski fakultet, Zagreb
Profili:
Marin Torti
(autor)
Alan Kovačević
(autor)
Anita Horvatić
(autor)
Vladimir Mrljak
(autor)
Ivana Rubić
(autor)
Petra Nižić
(autor)
Josipa Kuleš
(autor)
Ines Jović
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE