Naslov
Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine

Autori
Wasko, Joanna ; Fraczyk, Justyna ; Becht, Angelika ; Kaminski, Zbigniew ; Flinčec Grgac, Sandra ; Tarbuk, Anita ; Kaminska, Marta ; Dudek, Mariusz ; Gliscinska, Eulalia ; Draczynski, Zbigniew ; Kolesinska, Beata

Izvornik
Materials (1996-1944) 13 (2020), 14; 3079, 29

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
conjugate of polysaccharides with peptides ; alginate ; chitosan ; proline ; hydroxyproline derivatives ; mimetic of functional collagen fragments ; stable spatial structure

Sažetak
New materials that are as similar as possible in terms of structure and biology to the extracellular matrix (external environment) of cells are of great interest for regenerative medicine. Oligoproline and oligohydroxyproline derivatives (peptides 2–5) are potential mimetics of collagen fragments. Peptides 2–5 have been shown to be similar to the model collagen fragment (H-Gly-Hyp-Pro-Ala-Hyp-Pro- OH, 1) in terms of both their spatial structure and biological activity. In this study, peptides 2–5 were covalently bound to nonwovens based on chitosan and calcium alginate. Incorporation of the peptides was confirmed by Fourier transform -infrared (FT-IR) and zeta potential measurements. Biological studies (cell metabolic activity by using 3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) test and Live/Dead assay) proved that the obtained peptide-polysaccharide conjugates were not toxic to the endothelial cell line EA.hy 926. In many cases, the conjugates had a highly affirmative influence on cell proliferation. The results of this study show that conjugates of chitosan and calcium alginate with oligoproline and oligohydroxyproline derivatives have potential for use in regenerative medicine.

Izvorni jezik
Engleski

Znanstvena područja
Tekstilna tehnologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

Napomena
Lodz University of Technology

POVEZANOST RADA