Naslov
Pathogenicity of Campylobacter jejuni, C. upsaliensis and C. helveticus in the invertebrate disease model Galleria mellonella

Autori
Bojanić, Krunoslav ; Midwinter, Anne Camilla ; Biggs, Patrick Jon ; Marshall, Jonathan Craig ; Acke, Els ;

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Delegate Handbook of the 18th International Campylobacter, Helicobacter & Related Organisms Conference / On, Stephen - Palmerston North : CHRO Conference 2015, 2015, 38-38

ISBN
978-0-473-34060-5

Skup
18th International Campylobacter, Helicobacter & Related Organisms Conference

Mjesto i datum
Rotorua, Novi Zeland, 01.11.2015. - 05.11.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pathogenicity ; Campylobacter ; animal model ; zoonosis ; dogs ; cats ; Galleria mellonella ;

Sažetak
Campylobacter jejuni (CJ) is a well-established human pathogen but many other species in the genus have discordant or a lack of evidence of pathogenic potential. C. upsaliensis (CU) is also a recognized human pathogen and C. helveticus (CH), despite a high level of genetic similarity to CU, is not. The invertebrates have a high degree of functional and structural homology with the mammalian innate immune system and larvae of the Greater Wax Moth, Galleria mellonella, has been described as an animal model for CJ. This study aimed to compare the pathogenicity of CJ, CU and CH in this model. Thirty-one isolates of CJ, 19 of CU and 11 of CH were used for inoculation into the haemocoel via the pro-leg of 4, 596 larvae. Controls consisted of 313 phosphate-buffered saline inoculated and 306 un-inoculated larvae. Experiments were performed with (i) live bacteria in varying doses ; (ii) heat and freeze-thaw inactivated whole cells, insoluble and soluble cellular components ; (iii) room and 37°C incubating temperatures and, (iv) aerobic and H 2 -enriched microaerobic atmospheres. Technical and biological replicates were used and survival was monitored up to 8 days post-injection. Survival of larvae was dependent on the species of inoculum, bacterial dose, bacterial components/products and environmental conditions. Generally, species diff erences were more pronounced with live cell assays where CJ caused a higher and faster larval death rate than CU and CH. The observations of diff erences in the pathogenicity of Campylobacter species in this disease model correlate with observations from comparative epidemiological and genomic studies.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina

POVEZANOST RADA