Naslov
Suppression of experimental allergic encephalomyelitis by administration of myelin antigens

Autori
Taradi, Milan ; Kukolja-Taradi, Sunčana ; Župančić, Vera ; Andreis, Igor ; Lukinović-Škudar, Vesna

Izvornik
Periodicum biologorum. Supplement (0353-9164) 99 (1997), Suppl. 2; 26-26

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
multiple sclerosis; allergic enncephalomyelitis

Sažetak
Experimental allergic encephalomyelitis (EAE) is a autoimune demyelinating disease of central nervous system (CNS) and serves as a model for human demyelinating diseases, such as multiple sclerosis. EAE has been shown to be mediated by autoreactive T lymphocytes specific for myelin basic protein (MBP), but B cells and macrophages are also implicated in the disease process. We described here the suppression of clinical symptoms of EAE by administration of low doses of myelin antigens before of induction of disease. Rats of WVM strain were used in the experiments and EAE was induced by encephalytogeneic vaccine. The whole rat brain was homogenized and then emulsified with complete Freund, s antigen (CFA) in the volume ratio of 1:5. The total dose of 1 mL vaccine was injected at 10 sites (group 1). Animals ware examined daily for clinical symptoms and the severity of the disease was graduated from 1 to 5. Group 2 and 3 rats was treated with two injection of MBP or ovalbumin (OVA) respectively, 8 and 3 days prior to the immunization with brain tissue in CFA. The incidence of EAE in control mice (group 1) (70% ; 10/14) and in group treated with OA (group 3) (70% ; 9/13) was not statistically different. Only 1 of 15 rats (7% ; 1/15) in group 2 received MBA developed mild clinical signs of disease. The herein presented data show that treatment of rats before immunization with low doses of MBP, but not with unrelated OA, strongly reduces the clinical symptoms of EAE.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA