Naslov
An outbreak of human parainfluenza virus 3 (phylogenetic subcluster C5) infection among adults at a residential care facility for the disabled in Croatia, 2018
(An outbreak of human parainfluenza virus 3 (phylogenetic subcluster C5) infection among adults at a residential care facility for the disabled in Croatia, 2018)

Autori
Čivljak, Rok ; Košutić-Gulija, Tanja ; Slović, Anamarija ; Huljev, Eva ; Turčić, Nikolina ; Meštrović, Tomislav ; Vraneš, Jasmina ; Ljubin Sternak, Sunčanica

Izvornik
Intervirology (0300-5526) 62 (2019), 5-6; 174-181

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
human parainfluenza virus 3 ; pneumonia ; community residential care home ; multiplex PCR ; sequencing

Sažetak
Introduction: Although highly pertinent for children, outbreaks of human parainfluenza virus (HPIV) may cause up to 15% of all respiratory illnesses in adults and predispose them to serious adverse outcomes, with HPIV serotype 3 (HPIV3) being the most common. This study represents the first report of an HPIV3 outbreak among adults at a long-term health- care facility in Croatia. Methods: A retrospective study was conducted to investigate an outbreak of acute respiratory infection (ARI) at a single residential care facility for the disabled in Croatia. Demographic, epidemiological, and clinical data were collected for all residents, while hospitalized patients were appraised in detail by laboratory/radiological methods. Multiplex PCR for respiratory viruses and sequencing was performed. Partial HPIV3 HN 581 nt sequences were aligned with HPIV3 sequences from the GenBank database to conduct a phylogenetic analysis, where different bioinformatic approaches were employed. Results: In late June 2018, 5 of the 10 units at the facility were affected by the outbreak. Among the 106 residents, 23 (21.7%) developed ARI, and 6 (26.1%) of them were hospitalized. HPIV3 was identified in 18 (73%) of the residents and 5 (83%) of the hospitalized individuals. Isolated HPIV3 strains were classified within the phylogenetic subcluster C5 but grouped on 2 separate branches of the phylogenetic tree. During the entire outbreak period, none of the institution’s employees reported symptoms of ARI. Conclusions: Our study has shown that this health care-associated outbreak of HPIV3 infection could have been linked to multiple importation events. Preventive measures in curbing such incidents should be enforced vigorously.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA