Naslov
Stability and bioaccessibility during ex vivo digestion of glucoraphenin and glucoraphasatin from Matthiola incana (L.) R. Br.

Autori
Blažević, Ivica ; Đulović, Azra ; Burčul, Franko ; Popović, Marijana ; Montaut, Sabina ; Bilušić, Tea ; Vrca, Ivana ; Markić, Joško ; Ljubenkov, Ivica ; Ruščić, Mirko ; Rollin, Patrick

Izvornik
Journal of food composition and analysis (0889-1575) 90 (2020); 103483, 7

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Matthiola incana ; glucoraphenin ; desulfoglucoraphenin ; glucoraphasatin ; gastrointestinal digestion ; bioaccessibility

Sažetak
The glucosinolates (GSLs) of Matthiola incana were characterized and quantified as desulfoGSLs in different plant tissues (seeds, siliquae, stems, leaves, and flowers) using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-DAD-MS/MS). The major GSL detected in all plant parts was 4- (me- thylsulfinyl)but-3-enyl GSL (glucoraphenin, 1), followed by 4-(methylsulfanyl)but-3-enyl GSL (glucoraphasatin, 2), while indol-3- ylmethyl GSL (glucobrassicin, 3) was also found in the flowers. GSL 2 was found to be the dominant in the plants analysed after only 2 days of drying, contrary to all analyses performed after longer storage of plant material, thus suggesting an oxidation of 2 into 1. The stability of GSLs was monitored over a one-month period. The desulfo- counterpart of 1 was unstable in water, showing a dramatic decrease (ca 80%) during the observation time, with spontaneous conversion into cyclic thioimidate N-oxides. Bioaccessibility of GSLs through the simulated ex vivo gastrointestinal digestion was determined using human gastrointestinal juices. Glucoraphenin 1 showed high bioaccessibility in the gastric phase (> 90%), while both GSLs 1 and 2 were transformed in the intestinal phase.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Prehrambena tehnologija

POVEZANOST RADA