Naslov
High phylogenetic diversity of Gallibacterium anatis is correlated with low biosecurity measures and management practice on poultry farms

Autori
Lozica, Liča ; Kazazić, Snježana ; Gottstein, Željko

Izvornik
Avian pathology (0307-9457) 49 (2020), 5; 467-475

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Gallibacterium anatis ; rpoB ; phylogeny ; epidemiology ; poultry ; biosecurity

Sažetak
Gallibacterium anatis is considered one of the most common bacterial causative agents of reproductive tract disorders in poultry. In this study, phylogenetic analysis of partial rpoB sequences and biotyping using MALDI-TOF MS were done in order to investigate the genetic diversity of Gallibacterium isolates from thirteen farms with different biosecurity measures and management practices. Sampling was done as a part of regular monitoring, except for Farms 9-13 that were included in the study to represent extensive production system with lowest biosecurity levels. Pharyngeal and cloacal swabs were taken from live animals, while swabs from trachea, liver, peritoneum and oviduct were taken during necropsies. After cultivation and identification, strains from each farm were randomly selected for sequencing and biotyping. The results showed high level of heterogeneity among the isolates originating from farms with low biosecurity levels, unlike isolates from farms with higher biosecurity levels and proper management that were more closely related and clustered together. Such correlation was statistically significant. Low biosecurity levels enable horizontal transmission of the pathogens, as well as gene transfer. The results confirm the importance of adequate biosecurity measures and management on poultry farms as they greatly affect the genetic diversity of the pathogens. Therefore, implementation of basic biosecurity measures could help control the heterogeneity of Gallibacterium strains, which would alleviate control of the infection prevalence on farms through immunoprophylaxis and consequently improve poultry production. Also, the genetic diversity of Gallibacterium anatis on poultry farms could be a good bioindicator of management practice and biosecurity measures used.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA