Naslov
Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes

Autori
Zorić, Nataša ; Kopjar, Nevenka ; Vuković Rodriguez, Jadranka ; Tomić, Siniša ; Kosalec, Ivan

Izvornik
Acta pharmaceutica (1330-0075) 71 (2021), 1; 131-141

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
oleuropein ; hydroxytyrosol ; olive ; antioxidant activity ; DNA damage ; human lymphocytes ; comet assay

Sažetak
This study investigates antioxidant capacity and protective effects of phenolic compounds oleuropein (OLP) and hydroxytyrosol (HT), present in olive oil and olive leaves, against H2O2- induced DNA damage in human peripheral lymphocytes. Antioxidant potency was determined using the measurement of radical-scavenging activity (ABTS∙+ assay), ferric reducing power (FRAP assay) and cupric reducing antioxidant capacity (CUPRAC assay). Both substances were found to be potent antioxidant agents due to their free radical- scavenging activities. Antigenotoxic effects of oleuropein and hydroxytyrosol against H2O2-induced damage in human lymphocytes were evaluated in vitro by alkaline comet assay. At tested concentrations (1, 5, 10 μmol L–1), oleuropein and hydroxytyrosol did not induce a significant increase of primary DNA damage in comparison with the negative control. Pretreatment of human lymphocytes with each of the substances for 120 min produced a dose-dependent reduction of primary DNA damage in the tested cell type. Hydroxytyrosol showed a better protective effect against H2O2-induced DNA breaks than oleuropein which could be associated with their free radical- scavenging efficacy.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

Napomena
Rad je izveden unutar doktorskog rada kolegice
Nataše Zorić (HALMED) pod sumentorstvom prof. dr.
sc. I. Kosalca i prof. dr. sc. S. Tomića.

POVEZANOST RADA