Pregled bibliografske jedinice broj: 1055045
Nutritional stress in head and neck cancer originating cell lines: the sensitivity of the NRF2-NQO1 axis
Nutritional stress in head and neck cancer originating cell lines: the sensitivity of the NRF2-NQO1 axis // Cells, 8 (2019), 9; 1001, 28 doi:10.3390/cells8091001 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1055045 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Nutritional stress in head and neck cancer
originating cell lines: the sensitivity of the
NRF2-NQO1 axis
Autori
Milković, Lidija ; Tomljanović, Marko ; Čipak Gašparović, Ana ; Novak Kujundžić, Renata ; Šimunić, Dina ; Konjevoda, Paško ; Mojzeš, Anamarija ; Đaković, Nikola ; Žarković, Neven ; Gall Trošelj, Koraljka
Izvornik
Cells (2073-4409) 8
(2019), 9;
1001, 28
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
glucose deprivation ; glutamine deprivation ; viability ; proliferation ; ROS ; NRF2-NQO1 axis ; IMR-90 ; NQO1 transcript variants ; rs1800566 ; TP53 mutation
Sažetak
Nutritional stress disturbs the cellular redox- status, which is characterized by the increased generation of reactive oxygen species (ROS). The NRF2-NQO1 axis represents a protective mechanism against ROS. Its strength is cell type-specific. FaDu, Cal 27 and Detroit 562 cells differ with respect to basal NQO1 activity. These cells were grown for 48 hours in nutritional conditions (NC): (a) Low glucose–NC2, (b) no glucose, no glutamine– NC3, (c) no glucose with glutamine–NC4. After determining the viability, proliferation and ROS generation, NC2 and NC3 were chosen for further exploration. These conditions were also applied to IMR-90 fibroblasts. The transcripts/transcript variants of NRF2 and NQO1 were quantified and transcript variants were characterized. The proteins (NRF2, NQO1 and TP53) were analyzed by a western blot in both cellular fractions. Under NC2, the NRF2-NQO1 axis did not appear activated in the cancer cell lines. Under NC3, the NRF2- NQO1axis appeared slightly activated in Detroit 562. There are opposite trends with respect to TP53 nuclear signal when comparing Cal 27 and Detroit 562 to FaDu, under NC2 and NC3. The strong activation of the NRF2-NQO1 axis in IMR-90 resulted in an increased expression of catalytically deficient NQO1, due to NQO1*2/*2 polymorphism (rs1800566). The presented results call for a comprehensive exploration of the stress response in complex biological systems.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-4404 - NRF2 na raskrižju epigenetičkog modeliranja, metabolizma i proliferacije stanice raka (CrossEMPATICNRF2) (Gall-Trošelj, Koraljka, HRZZ - 2016-06) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
KBC "Sestre Milosrdnice"
Profili:
Koraljka Gall Trošelj
(autor)
Nikola Đaković
(autor)
Anamarija Mojzeš
(autor)
Ana Čipak Gašparović
(autor)
Renata Novak Kujundžić
(autor)
Lidija Milković
(autor)
Paško Konjevoda
(autor)
Marko Tomljanović
(autor)
Neven Žarković
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- MEDLINE
Uključenost u ostale bibliografske baze podataka::
- EMBASE (Excerpta Medica)