Naslov
Hyperthermic modulation of resistance to cis-diammine-dichloroplatinum(II) in human larynx carcinoma cells

Autori
Beketić-Orešković, Lidija ; Jakšić, Milko ; Orešković, Slavko ; Osmak, Maja

Izvornik
International journal of hyperthermia (0265-6736) 13 (1997), 2; 205-214

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cisplatin; hyperthermia; reversal of drug resistance; human tumor cells

Sažetak
The aim of the present study was to examine the effect of hyperthermia on cisplatin resistance in two sublines of human larynx carcinoma HEp2 cells. Hyperthermia was tumouricidal for both parental and cisplatin cells, but cisplatin resistant cells were more sensitive to the heat at 43 and 44oC. In combined cisplatin hyperthermia treatment, heat chemosensitization was observed for parental, as well as for cis-platin cells. At 42oC and at 43oC, the thermal enhacement ratio (TER) for cisplatin toxicity was higher in resistant than in parental cells. The heating at 42oC decreased the resistance factors in CA3 and CK2 cells from 2.2 and 3.5 to 1.8 and 2.2 respectively. At 43oC, the resistance factors were decreased to 1.7 for CA3 cells and 2.0 for CK2 cells. The heating at 43oC increased intracellular platinum accumulation in all cell lines examined. This effect was more pronounced in resistant than in parental cells, causing complete reversal of the reduced platinum accumulation found at 37oC in resistant cells. In conclusion, the resistance to cisplatin was partially reversed by hyperthermia in CA3 and CK2 cells. Increased drug accumulation was one of the mechanisms involved in hyperthermic potentiation of cisplatin toxicity and partial reversal of cisplatin resistance in human larynx carcinoma HEp2 cells.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija

POVEZANOST RADA