Naslov
Inhibition of miR-223 expression using a sponge strategy decreases restenosis in rat injured carotids

Autori
M’baya-Moutoula, Eleonore ; Marchand, Alexandre ; Six, Isabelle ; Bahrar, Noura ; Celic, Tanja ; Mougenot, Nathalie ; Maitrias, Pierre ; Massy, Ziad A. ; Lompreh, Anne-Marie ; Metzinger, Laurent ; Meuth, Valérie Metzinger-Le

Izvornik
Current Vascular Pharmacology (1570-1611) 17 (2019), 5; 5, 10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Restenosis ; miR-223 ; microRNA ; rat carotid ; vascular smooth muscle cells

Sažetak
Objective: Restenosis is a frequent complication of angioplasty. It consists of a neointimal hyperplasia resulting from progression and migration of vascular smooth muscle cells (VSMC) into the vessel lumen. microRNA miR-223 has recently been shown to be involved in cardiovascular diseases including atherosclerosis, vascular calcification and arterial thrombosis. In this study, our aim was to assess the impact of miR-223 modulation on restenosis in a rat model of carotid artery after balloon injury. Methods: The over and down-expression of miR-223 was induced by adenoviral vectors, containing either a pre-miR-223 sequence allowing artificial miR-223 expression or a sponge sequence, trapping the native microRNA, respectively. Restenosis was quantified on stained rat carotid sections. Results: In vitro, three mRNA (Myocyte Enhancer Factor 2C (MEF2C), Ras homolog gene family, member B (RhoB) and Nuclear factor 1 A-type (NFIA)) reported as miR-223 direct targets and known to be implicated in VSMC differentiation and contractility were studied by RT-qPCR. Our findings showed that down-expression of miR-223 significantly reduced neointimal hyperplasia by 44% in carotids, and was associated with a 2-3- fold overexpression of MEF2C, RhoB and NFIA in a murine monocyte macrophage cell line, RAW 264.7 cells. Conclusion: Down-regulating miR-223 could be a potential therapeutic approach to prevent restenosis after angioplasty.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA