Naslov
Expression of ferritin light and heavy chains in rat liver: sex and age differences

Autori
Pavić, Mirela ; Turčić, Petra ; Antolić, Nikolina ; Karaica, Dean ; Vrhovac Madunić, Ivana ; Micek, Vedran ; Breljak, Davorka ; Ljubojević, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts "9th Croatian Congress of Pharmacology with International Participation" / - Zagreb, 2019, 137-137

Skup
9. hrvatski kongres farmakologije = 9th Croatian Congress of Pharmacology

Mjesto i datum
Zagreb, Hrvatska, 25.09.2019. - 28.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
ferritin, sex differences, age differences, liver, rat

Sažetak
Introduction: The ferritin complex consists of two protein chains structured in a 24 subunit nanocage for iron storage. Very early research on liver in experimental animals found sex differences in favor of females in the amount of iron, which was later associated with sex differences in the ferritin amount in the liver as an iron storage organ. Materials and Methods: We used specific antibodies for two ferritin subunits followed by immunochemical methods for sex and age differences in the expression of light and heavy subunits of ferritin in the liver of three-month and two-year old Wistar rats together with gonadectomized three-month animals for steroid hormone influence determination. Distribution of ferritin subunits inside the organ was obtained by immunohistochemistry, while differences in the expression of individual subunits were determined by western analysis. Results: The results of immunochemical analyses confirmed sex differences in both ferritin subunits and these remained with age, while the amount of ferritin in liver increased with age. Gonadectomy showed a positive effect of estrogen on the expression of both chains and a negative of testosterone. Both ferritin subunits displayed an overlapping distribution in liver cells, which was most pronounced in hepatocytes around the central veins. Conclusion: We can conclude that both ferritin subunits were expressed in the liver and showed sex differences influenced by steroid hormones and age differences which may be correlated with an enlarged amount of stored iron and thus with a great threat of increased oxidative stress crucial to the pathophysiological mechanisms of the aging process.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Veterinarska medicina, Farmacija

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