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Pregled bibliografske jedinice broj: 1049465

. Osteoclasts Derive Predominantly from Bone Marrow-Resident CX3CR1+ Precursor Cells in Homeostasis, whereas Circulating CX3CR1+ Cells Contribute to Osteoclast Development during Fracture Repair


Novak, Sanja; Roeder, E.; Kalinowski, J.; Jastrzebski, S.; Aguila, HL; Lee, SK; Kalajzic, Ivo; Lorenzo, JA
. Osteoclasts Derive Predominantly from Bone Marrow-Resident CX3CR1+ Precursor Cells in Homeostasis, whereas Circulating CX3CR1+ Cells Contribute to Osteoclast Development during Fracture Repair // Journal of immunology, 204 (2020), 4; 868-878 doi:10.4049/jimmunol.1900665 (međunarodna recenzija, članak, znanstveni)


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Naslov
. Osteoclasts Derive Predominantly from Bone Marrow-Resident CX3CR1+ Precursor Cells in Homeostasis, whereas Circulating CX3CR1+ Cells Contribute to Osteoclast Development during Fracture Repair

Autori
Novak, Sanja ; Roeder, E. ; Kalinowski, J. ; Jastrzebski, S. ; Aguila, HL ; Lee, SK ; Kalajzic, Ivo ; Lorenzo, JA

Izvornik
Journal of immunology (0022-1767) 204 (2020), 4; 868-878

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
OCP express CX3CR1 ; osteoclasts ; bone marrow ; BM macrophage/monocyte ; OC progenitor

Sažetak
Osteoclasts (OC) originate from either bone marrow (BM)–resident or circulating myeloid OC progenitors (OCP) expressing the receptor CX3CR1. Multiple lines of evidence argue that OCP in homeostasis and inflammation differ. We investigated the relative contributions of BM-resident and circulating OCP to osteoclastogenesis during homeostasis and fracture repair. Using CX3CR1-EGFP/TRAP tdTomato mice, we found CX3CR1 expression in mononuclear cells, but not in multinucleated TRAP+ OC. However, CX3CR1-expressing cells generated TRAP+ OC on bone within 5 d in CX3CR1CreERT2/Ai14 tdTomato reporter mice. To define the role that circulating cells play in osteoclastogenesis during homeostasis, we parabiosed TRAP tdTomato mice (CD45.2) on a C57BL/6 background with wild-type (WT) mice (CD45.1). Flow cytometry (CD45.1/45.2) demonstrated abundant blood cell mixing between parabionts after 2 wk. At 4 wk, there were numerous tdTomato+ OC in the femurs of TRAP tdTomato mice but almost none in WT mice. Similarly, cultured BM stimulated to form OC demonstrated multiple fluorescent OC in cell cultures from TRAP tdTomato mice, but not from WT mice. Finally, flow cytometry confirmed low-level engraftment of BM cells between parabionts but significant engraftment in the spleens. In contrast, during fracture repair, we found that circulating CX3CR1+ cells migrated to bone, lost expression of CX3CR1, and became OC. These data demonstrate that OCP, but not mature OC, express CX3CR1 during both homeostasis and fracture repair. We conclude that, in homeostasis mature OC derive predominantly from BM-resident OCP, whereas during fracture repair, circulating CX3CR1+ cells can become OC.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Profili:

Avatar Url Ivo Kalajzić (autor)

Avatar Url Sanja Novak (autor)

Poveznice na cjeloviti tekst rada:

doi www.jimmunol.org doi.org

Citiraj ovu publikaciju:

Novak, Sanja; Roeder, E.; Kalinowski, J.; Jastrzebski, S.; Aguila, HL; Lee, SK; Kalajzic, Ivo; Lorenzo, JA
. Osteoclasts Derive Predominantly from Bone Marrow-Resident CX3CR1+ Precursor Cells in Homeostasis, whereas Circulating CX3CR1+ Cells Contribute to Osteoclast Development during Fracture Repair // Journal of immunology, 204 (2020), 4; 868-878 doi:10.4049/jimmunol.1900665 (međunarodna recenzija, članak, znanstveni)
Novak, S., Roeder, E., Kalinowski, J., Jastrzebski, S., Aguila, H., Lee, S., Kalajzic, I. & Lorenzo, J. (2020) . Osteoclasts Derive Predominantly from Bone Marrow-Resident CX3CR1+ Precursor Cells in Homeostasis, whereas Circulating CX3CR1+ Cells Contribute to Osteoclast Development during Fracture Repair. Journal of immunology, 204 (4), 868-878 doi:10.4049/jimmunol.1900665.
@article{article, author = {Novak, Sanja and Roeder, E. and Kalinowski, J. and Jastrzebski, S. and Aguila, HL and Lee, SK and Kalajzic, Ivo and Lorenzo, JA}, year = {2020}, pages = {868-878}, DOI = {10.4049/jimmunol.1900665}, keywords = {OCP express CX3CR1, osteoclasts, bone marrow, BM macrophage/monocyte, OC progenitor}, journal = {Journal of immunology}, doi = {10.4049/jimmunol.1900665}, volume = {204}, number = {4}, issn = {0022-1767}, title = {. Osteoclasts Derive Predominantly from Bone Marrow-Resident CX3CR1+ Precursor Cells in Homeostasis, whereas Circulating CX3CR1+ Cells Contribute to Osteoclast Development during Fracture Repair}, keyword = {OCP express CX3CR1, osteoclasts, bone marrow, BM macrophage/monocyte, OC progenitor} }
@article{article, author = {Novak, Sanja and Roeder, E. and Kalinowski, J. and Jastrzebski, S. and Aguila, HL and Lee, SK and Kalajzic, Ivo and Lorenzo, JA}, year = {2020}, pages = {868-878}, DOI = {10.4049/jimmunol.1900665}, keywords = {OCP express CX3CR1, osteoclasts, bone marrow, BM macrophage/monocyte, OC progenitor}, journal = {Journal of immunology}, doi = {10.4049/jimmunol.1900665}, volume = {204}, number = {4}, issn = {0022-1767}, title = {. Osteoclasts Derive Predominantly from Bone Marrow-Resident CX3CR1+ Precursor Cells in Homeostasis, whereas Circulating CX3CR1+ Cells Contribute to Osteoclast Development during Fracture Repair}, keyword = {OCP express CX3CR1, osteoclasts, bone marrow, BM macrophage/monocyte, OC progenitor} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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