Naslov
Human amniotic fluid stem cells attract osteoprogenitor cells in bone healing

Autori
Basile, Mariangela ; Marchegiani, Francesco ; Novak, Sanja ; Kalajzic, Ivo ; Di Pietro, Roberta

Izvornik
Journal of cellular physiology (0021-9541) 235 (2020), 5; 4643-4654

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
bone ; mice ; transgenic ; regenerative medicine ; stem cell transplantation ; transgenic ; IN-VIVO

Sažetak
Current treatments of large bone defects are based on autologous or allogenic bone transplantation. Human amniotic fluid stem cells (hAFSCs) were evaluated for their potential in bone regenerative medicine. In this study, hAFSCs were transduced with lentiviral vector harboring red fluorescent protein to investigate their role in the regeneration of critical-size bone defects in calvarial mouse model. To distinguish donor versus recipient cells, a transgenic mouse model carrying GFP fluorescent reporter was used as recipient to follow the fate of hAFSCs transplanted in vivo into Healos (R) scaffold. Our results showed that transduced hAFSCs can be tracked in vivo directly at the site of transplantation. The presence of GFP positive cells in the scaffold at 3 and 6 weeks after transplantation indicates that donor hAFSCs can recruit host cells during the repair process. These observations help clarify the role of hAFSCs in bone tissue repair.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA