Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1049445

Skeletal phenotype of the neuropeptide Y knockout mouse


Wee, Natalie K.Y.; Sinder, Benjamin P.; Novak, Sanja; Wang, Xi; Stoddard, Chris; Matthews, Brya G.; Kalajzic, Ivo
Skeletal phenotype of the neuropeptide Y knockout mouse // Neuropeptides, 73 (2019), 78-88 doi:10.1016/j.npep.2018.11.009 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1049445 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Skeletal phenotype of the neuropeptide Y knockout mouse

Autori
Wee, Natalie K.Y. ; Sinder, Benjamin P. ; Novak, Sanja ; Wang, Xi ; Stoddard, Chris ; Matthews, Brya G. ; Kalajzic, Ivo

Izvornik
Neuropeptides (0143-4179) 73 (2019); 78-88

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Neuropeptide Y (NPY) ; Conditional mouse ; Bone ; Cortical bone ; Bone strength

Sažetak
Neuropeptide Y (NPY) is involved in multiple processes such as behavior, energy and bone metabolism. Previous studies have relied on global NPY depletion to examine its effects on bone. However, this approach is unable to distinguish the central or local source of NPY influencing bone. Our aim was to identify which cells within the skeleton express Npy and establish a model that will enable us to differentiate effects of NPY derived from different cell types. We have generated the NPY floxed (NPYflox) mice using CRISPR technology. By crossing the NPYflox mice with Hypoxanthine Phosphoribosyltransferase 1 (Hprt)-cre to generate a global knockout, we were able to validate and confirm loss of Npy transcript and protein in our global NPYKO. Global deletion of NPY results in a smaller femoral cortical cross-sectional area (-12%) and reduced bone strength (-18%) in male mice. In vitro, NPY-deficient bone marrow stromal cells (BMSCs) showed increase in osteogenic differentiation detected by increases in alkaline phosphatase staining and bone sialoprotein and osteocalcin expression. Despite both sexes presenting with increased adiposity, female mice had no alterations in bone mass, suggesting that NPY may have sex-specific effects on bone. In this study we identified Npy expression in the skeleton and examined the effect of global NPY depletion to bone mass. The differential impact of NPY deletion in cortical and cancellous compartments along with differences in phenotypes between in vitro and in vivo, highlights the complex nature of NPY signaling, indicative of distinct sources that can be dissected in the future using this NPYflox model.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Profili:

Avatar Url Ivo Kalajzić (autor)

Avatar Url Sanja Novak (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com doi.org

Citiraj ovu publikaciju:

Wee, Natalie K.Y.; Sinder, Benjamin P.; Novak, Sanja; Wang, Xi; Stoddard, Chris; Matthews, Brya G.; Kalajzic, Ivo
Skeletal phenotype of the neuropeptide Y knockout mouse // Neuropeptides, 73 (2019), 78-88 doi:10.1016/j.npep.2018.11.009 (međunarodna recenzija, članak, znanstveni)
Wee, N., Sinder, B., Novak, S., Wang, X., Stoddard, C., Matthews, B. & Kalajzic, I. (2019) Skeletal phenotype of the neuropeptide Y knockout mouse. Neuropeptides, 73, 78-88 doi:10.1016/j.npep.2018.11.009.
@article{article, author = {Wee, Natalie K.Y. and Sinder, Benjamin P. and Novak, Sanja and Wang, Xi and Stoddard, Chris and Matthews, Brya G. and Kalajzic, Ivo}, year = {2019}, pages = {78-88}, DOI = {10.1016/j.npep.2018.11.009}, keywords = {Neuropeptide Y (NPY), Conditional mouse, Bone, Cortical bone, Bone strength}, journal = {Neuropeptides}, doi = {10.1016/j.npep.2018.11.009}, volume = {73}, issn = {0143-4179}, title = {Skeletal phenotype of the neuropeptide Y knockout mouse}, keyword = {Neuropeptide Y (NPY), Conditional mouse, Bone, Cortical bone, Bone strength} }
@article{article, author = {Wee, Natalie K.Y. and Sinder, Benjamin P. and Novak, Sanja and Wang, Xi and Stoddard, Chris and Matthews, Brya G. and Kalajzic, Ivo}, year = {2019}, pages = {78-88}, DOI = {10.1016/j.npep.2018.11.009}, keywords = {Neuropeptide Y (NPY), Conditional mouse, Bone, Cortical bone, Bone strength}, journal = {Neuropeptides}, doi = {10.1016/j.npep.2018.11.009}, volume = {73}, issn = {0143-4179}, title = {Skeletal phenotype of the neuropeptide Y knockout mouse}, keyword = {Neuropeptide Y (NPY), Conditional mouse, Bone, Cortical bone, Bone strength} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font