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Pregled bibliografske jedinice broj: 1048515

Microwave-Assisted versus Conventional Isolation of Glucosinolate Degradation Products from Lunaria annua L. and Their Cytotoxic Activity

Blažević, Ivica; Đulović, Azra; Čikeš Čulić, Vedrana; Popović, Marijana, Guillot, Xavier, Burčul, Franko; Rollin, Patrick
Microwave-Assisted versus Conventional Isolation of Glucosinolate Degradation Products from Lunaria annua L. and Their Cytotoxic Activity // Biomolecules, 10 (2020), 215; 1-11 doi:10.3390/biom10020215 (međunarodna recenzija, članak, znanstveni)

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Naslov
Microwave-Assisted versus Conventional Isolation of Glucosinolate Degradation Products from Lunaria annua L. and Their Cytotoxic Activity

Autori
Blažević, Ivica ; Đulović, Azra ; Čikeš Čulić, Vedrana ; Popović, Marijana, Guillot, Xavier, Burčul, Franko ; Rollin, Patrick

Izvornik
Biomolecules (2218-273X) 10 (2020), 215; 1-11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lunaria annua ; glucosinolates ; isothiocyanates ; microwave-assisted isolation ; cytotoxic activity ; human lung cancer cell line A549 ; breast cancer cell line MDA-MB-231

Sažetak
Glucosinolates (GSLs) from Lunaria annua L. seeds were analyzed qualitatively and quantitatively by their desulfo counterparts using UHPLC-DAD-MS/MS technique and by their volatile breakdown products, isothiocyanates (ITCs), using GC-MS technique. GSL breakdown products were obtained by conventional techniques (hydrodistillation in a Clevenger type apparatus (HD), CH2Cl2 extraction after myrosinase hydrolysis (EXT) for 24 h) as well as by modern techniques, microwave-assisted distillation (MAD) and microwave hydrodiffusion and gravity (MHG). Seven GSLs were identified as follows: isopropyl GSL (1), sec-butyl GSL (2), 5-(methylsulfinyl)pentyl GSL (3), 6-(methylsulfinyl)hexyl GSL (4), 5-(methylsulfanyl)pentyl GSL (5), 6-(methylsulfanyl)hexyl GSL (6), and benzyl GSL (7). Additionally, pent-4-enyl- and hex-5-enyl ITCs were detected in the volatile extracts. However, their corresponding GSLs were not detected using UHPLC-DAD-MS/MS. Thus, they are suggested to be formed during GC-MS analysis via thermolysis of 5-(methylsulfinyl)pentyl- and 6-(methylsulfinyl)hexyl ITCs, respectively. Volatile isolates were tested for their cytotoxic activity using MTT assay. EXT and MHG showed the best cytotoxic activity against human lung cancer cell line A549 during an incubation time of 72 h (IC50 18.8, and 33.5 μg/mL, respectively), and against breast cancer cell line MDA-MB-231 after 48 h (IC50 6.0 and 11.8 μg/mL, respectively). These activities can be attributed to the ITCs originating from 3 and 4.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija



POVEZANOST RADA

Projekti:
HRZZ-IP-2016-06-1316 - Biljke kao izvor bioaktivnih sumporovih spojeva te njihova sposobnost hiperakumulacije metala (BioSMe) (Blažević, Ivica, HRZZ - 2016-06) ( CroRIS)

Ustanove:
Kemijsko-tehnološki fakultet, Split,
Medicinski fakultet, Split

Profili:

Avatar Url Azra Đulović (autor)

Avatar Url Marijana Popovic (autor)

Avatar Url Vedrana Čikeš Čulić (autor)

Avatar Url Ivica Blažević (autor)

Avatar Url Franko Burčul (autor)

Poveznice na cjeloviti tekst rada:

doi doi.org

Citiraj ovu publikaciju:

Blažević, Ivica; Đulović, Azra; Čikeš Čulić, Vedrana; Popović, Marijana, Guillot, Xavier, Burčul, Franko; Rollin, Patrick
Microwave-Assisted versus Conventional Isolation of Glucosinolate Degradation Products from Lunaria annua L. and Their Cytotoxic Activity // Biomolecules, 10 (2020), 215; 1-11 doi:10.3390/biom10020215 (međunarodna recenzija, članak, znanstveni)
Blažević, I., Đulović, A., Čikeš Čulić, V., Popović, Marijana, Guillot, Xavier, Burčul, Franko & Rollin, P. (2020) Microwave-Assisted versus Conventional Isolation of Glucosinolate Degradation Products from Lunaria annua L. and Their Cytotoxic Activity. Biomolecules, 10 (215), 1-11 doi:10.3390/biom10020215.
@article{article, author = {Bla\v{z}evi\'{c}, Ivica and \DJulovi\'{c}, Azra and \v{C}ike\v{s} \v{C}uli\'{c}, Vedrana and Rollin, Patrick}, year = {2020}, pages = {1-11}, DOI = {10.3390/biom10020215}, keywords = {Lunaria annua, glucosinolates, isothiocyanates, microwave-assisted isolation, cytotoxic activity, human lung cancer cell line A549, breast cancer cell line MDA-MB-231}, journal = {Biomolecules}, doi = {10.3390/biom10020215}, volume = {10}, number = {215}, issn = {2218-273X}, title = {Microwave-Assisted versus Conventional Isolation of Glucosinolate Degradation Products from Lunaria annua L. and Their Cytotoxic Activity}, keyword = {Lunaria annua, glucosinolates, isothiocyanates, microwave-assisted isolation, cytotoxic activity, human lung cancer cell line A549, breast cancer cell line MDA-MB-231} }
@article{article, author = {Bla\v{z}evi\'{c}, Ivica and \DJulovi\'{c}, Azra and \v{C}ike\v{s} \v{C}uli\'{c}, Vedrana and Rollin, Patrick}, year = {2020}, pages = {1-11}, DOI = {10.3390/biom10020215}, keywords = {Lunaria annua, glucosinolates, isothiocyanates, microwave-assisted isolation, cytotoxic activity, human lung cancer cell line A549, breast cancer cell line MDA-MB-231}, journal = {Biomolecules}, doi = {10.3390/biom10020215}, volume = {10}, number = {215}, issn = {2218-273X}, title = {Microwave-Assisted versus Conventional Isolation of Glucosinolate Degradation Products from Lunaria annua L. and Their Cytotoxic Activity}, keyword = {Lunaria annua, glucosinolates, isothiocyanates, microwave-assisted isolation, cytotoxic activity, human lung cancer cell line A549, breast cancer cell line MDA-MB-231} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Emerging Sources Citation Index (ESCI)
  • Scopus
  • MEDLINE

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