Naslov
Adhesion of Campylobacter jejuni Is Increased in Association with Foodborne Bacteria

Autori
Klančnik, Anja ; Gobin, Ivana ; Jeršek, Barbara ; Smole Možina, Sonja ; Vučković, Darinka ; Tušek Žnidarič, Magda ; Abram, Maja

Izvornik
Microorganisms (2076-2607) 8 (2020); 201, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
adhesion ; abiotic/biotic surface ; Campylobacter jejuni ; co-culture ; survival ; pathogenicity

Sažetak
The aim of this study was to evaluate Campylobacter jejuni NTCT 11168 adhesion to abiotic and biotic surfaces when grown in co- culture with Escherichia coli ATCC 11229 and/or Listeria monocytogenes 4b. Adhesion of C. jejuni to polystyrene and to Caco-2 cells and Acanthamoeba castellanii was lower for at least 3 log CFU/mL compared to E. coli and L. monocytogenes. Electron micrographs of ultrathin sections revealed interactions of C. jejuni with host cells. In co-culture with E. coli and L. monocytogenes, adhesion of C. jejuni to all tested surfaces was significantly increased for more than 1 log CFU/mL. There was 10% higher aggregation for C. jejuni than for other pathogens, and high co- aggregation of co- cultures of C. jejuni with E. coli and L. monocytogenes. These data show that C. jejuni in co-cultures with E. coli and L. monocytogenes present significantly higher risk than C. jejuni as mono-cultures, which need to be taken into account in risk evaluation. C. jejuni adhesion is a prerequisite for their colonization, biofilm formation, and further contamination of the environment. C. jejuni survival under adverse conditions as a factor in their pathogenicity and depends on their adhesion to different surfaces, not only as individual strains, but also in co- cultures with other bacteria like E. coli and L. monocytogenes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne tehničke znanosti, Kliničke medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA