Naslov
In vivo assessment of genotoxicity in buccal cells of children undergoing tooth restoration

Autori
Gavić, Lidia ; Goršeta, Kristina ; Glavina, Domagoj ; Želježić, Davor ; Galić, Nada ; Tadin, Antonija

Izvornik
Central European journal of public health (1210-7778) 27 (2019), 4; 312-319

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Restorative dentistry ; Dental materials ; Genotoxicity test ; DNA damage ; Glass ionomer cements ; Compomers

Sažetak
Objectives: Whereas dental materials came in direct or close contact with oral tissue, it is a great concern about the biocompatibility of those materials. This study was performed to evaluate possible DNA damage to buccal cells exposed to dental materials. Methods: This prospective, longitudinal clinical study was conducted over a three months period. Class II restorations were placed in 60 young patients with no previous filling using one of three tested dental materials (two glass ionomers ; Ketac Molar and Ionofil Molar and one compomer material ; Twinky Star). DNA damage was analysed by micronucleus assays, in buccal exfoliated epithelial cells. Results: In patients treated with Ketac Molar, a significant frequency of micronuclei (p = 0.027) and binucleated cells in samples taken 30 days following restoration (p = 0.029) was confirmed. In patients treated with Twinky Star, a statistically significant increase in the number of binucleated cells in samples taken after 7 and 30 days following restoration (p = 0.001 and p < 0.001, respectively) was observed. In all samples collected 90 days after treatment, there was no statistical difference in the number of any cell changes. Conclusion: In this study long-term biocompatibility of tested materials was confirmed. Glass ionomers and compomers are widely used materials in paediatric dentistry, and this study has proved their safety for usage in children.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

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