Pregled bibliografske jedinice broj: 1043933
Activation of brown adipose tissue in diet-induced thermogenesis is GC-C dependent
Activation of brown adipose tissue in diet-induced thermogenesis is GC-C dependent // Pflügers Archiv, 472 (2020), 405-417 doi:10.1007/s00424-020-02347-8 (međunarodna recenzija, članak, znanstveni)
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Naslov
Activation of brown adipose tissue in diet-induced thermogenesis is GC-C dependent
Autori
Habek, Nikola ; Dobrivojević Radmilović, Marina ; Kordić, Milan ; Ilić, Katarina ; Grgić, Sandra ; Farkaš, Vladimir ; Bagarić, Robert ; Škokić, Siniša ; Švarc, Alfred ; Dugandžić, Aleksandra
Izvornik
Pflügers Archiv (0031-6768) 472
(2020);
405-417
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Uroguanylin ; Hypothalamus ; Male vs female ; PET-CT ; MRI ; Infrared thermography
Sažetak
Uroguanylin (UGN) is released from the intestine after a meal. When applied in brain ventricles, UGN increases expression of markers of thermogenesis in brown adipose tissue (BAT). Therefore, we determine the effects of its receptor, guanylate cyclase C (GC-C), on mouse interscapular BAT (iBAT) activity during diet-induced thermogenesis (DIT). The activation of iBAT after a meal is diminished in GC-C KO mice, decreased in female wild type (WT) mice, and abolished in old WT animals. The activation of iBAT after a meal is the highest in male WT animals which leads to an increase in GC-C expression in the hypothalamus, an increase in iBAT volume by aging, and induction of iBAT markers of thermogenesis. In contrast to iBAT activation after a meal, iBAT activation after a cold exposure could still exist in GC-C KO mice and it is significantly higher in female WT mice. The expression of GC-C in the proopiomelanocortin neurons of the arcuate nucleus of the hypothalamus but not in iBAT suggests central regulation of iBAT function. The iBAT activity during DIT has significantly reduced in old mice but an intranasal application of UGN leads to an increase in iBAT activity in a dose- dependent manner which is in strong negative correlation to glucose concentration in blood. This activation was not present in GC-CKOmice. Our results suggest the physiological role of GC-C on the BAT regulation and its importance in the regulation of glucose homeostasis and the development of new therapy for obesity and insulin resistance.
Izvorni jezik
Engleski
Znanstvena područja
Fizika, Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
HRZZ-IP-2018-01-7416 - Funkcija novo-otkrivenog proteina u mozgu, urogvanilina, od stanične fiziologije do ljudskog zdravlja (FURNACE/FURNACE) (Dugandžić, Aleksandra, HRZZ - 2018-01) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb
Profili:
Siniša Škokić (autor)
Katarina Ilić (autor)
Robert Bagarić (autor)
Marina Radmilović (autor)
Vladimir Farkaš (autor)
Alfred Švarc (autor)
Aleksandra Sinđić (autor)
Nikola Habek (autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE