Sterigmatocystin moderately induces oxidative stress in male Wistar rats after short-term oral treatment

Rašić, Dubravka; Jakšić, Daniela; Hulina Tomašković, Andrea; Kifer, Domagoj; Kopjar, Nevenka; Rumora, Lada; Želježić, Davor; Peraica, Maja; Šegvić Klarić, Maja

doi:10.1007/s12550-019-00382-8

Pregled bibliografske jedinice broj: 1037941

Sterigmatocystin moderately induces oxidative stress in male Wistar rats after short-term oral treatment

Rašić, Dubravka; Jakšić, Daniela; Hulina Tomašković, Andrea; Kifer, Domagoj; Kopjar, Nevenka; Rumora, Lada; Želježić, Davor; Peraica, Maja; Šegvić Klarić, Maja

Sterigmatocystin moderately induces oxidative stress in male Wistar rats after short-term oral treatment // Mycotoxin research, 36 (2020), 181-191 doi:10.1007/s12550-019-00382-8 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1037941 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Sterigmatocystin moderately induces oxidative stress in male Wistar rats after short-term oral treatment

Autori
Rašić, Dubravka ; Jakšić, Daniela ; Hulina Tomašković, Andrea ; Kifer, Domagoj ; Kopjar, Nevenka ; Rumora, Lada ; Želježić, Davor ; Peraica, Maja ; Šegvić Klarić, Maja

Izvornik
Mycotoxin research (0178-7888) 36 (2020); 181-191

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
sterigmatocystin ; malondialdehyde ; heat shock proteins ; DNA damage ; comet assay

Sažetak
This study aimed to explore involvement of oxidative stress in sterigmatocystin (STC) toxicity in male Wistar rats. Animals were orally treated with a single STC dose (10, 20 and 40 mg/kg b.w.). Short-term treatment resulted in moderate oxidative stress determined by a significant increase of malondialdehyde (MDA ; all STC doses) and catalase (CAT ; 10 mg/kg b.w.) in plasma, a decrease of glutathione peroxidase (GPx ; 20 and 40 mg/kg b.w.) in the liver, and increase of MDA and superoxide dismutase (SOD) in kidneys (all STC doses). Heat shock protein (Hsp27 and Hsp70) expression was determined by Western blotting in rat liver and kidneys. Hsp27 expression was downregulated by STC, particularly in the liver (40 mg/kg b.w.). The lowest STC dose elevated the expression of Hsp70 in both liver and kidneys, while an increase in STC doses restored Hsp70 expression to control. Alterations in expressions of Hsp27 and Hsp70 could be only partially associated with oxidative stress. STC provoked a significant DNA damage in both liver and kidneys (alkaline comet assay), but the liver was more affected by a broader spectrum of DNA lesions. Oxidative DNA damage (hOGG1-modified comet assay) contribute to the overall mechanism of STC-induced DNA damage in both organs, but kidneys in general seem to be more susceptible to oxidative stress upon short-term exposure to sublethal doses of STC.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita, Farmacija

POVEZANOST RADA

Projekti:
HRZZ-IP-2014-09-5982 - Štetni učinci pojedinačnih i kombiniranih mikotoksina Aspergillus vrsta (MycotoxA) (Šegvić Klarić, Maja, HRZZ - 2014-09) ( CroRIS)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb

Profili:

Domagoj Kifer (autor)