Naslov
Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells

Autori
Murati, Teuta ; Miletić, Marina ; Kolarić, Josipa ; Lovrić, Vanja ; Bursać Kovačević, Danijela ; Putnik, Predrag ; Landeka Jurčević, Irena ; Đikić, Domagoj ; Dragović-Uzelac, Verica ; Kmetič, Ivana

Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 70 (2019), 4; 303-309

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
blackthorn ; cytotoxicity ; Hep G2 ; kenacid blue ; neutral red ; ROS

Sažetak
Prunus spinosa L. (blackthorn) is used in traditional medicine as a remedy for various diseases. To establish its anticancer properties, we exposed human liver cancer cells (Hep G2) to a range of blackthorn flower extract concentrations (10-200 μg/mL) and determined cytotoxic activity with the neutral red and kenacid blue methods after 24, 48, and 72 h of incubation. Statistically significant inhibitory effects on Hep G2 cellular proliferation were observed at concentrations above 50 μg/mL (p<0.001–0.05). Cell viability was lower when determined with neutral red than kenacid blue method. In addition, we evaluated antioxidant/ prooxidant effects of the blackthorn flower extract by measuring reactive oxygen species (ROS), and the results confirmed its prooxidant behaviour within the applied concentration range. Flow cytometry determined primarily necrotic and apoptotic cell death, which provides additional evidence of its cytotoxic effect on liver carcinoma.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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