Naslov
BSA‐Polysaccharide Interactions at Negatively Charged Electrode Surface. Effects of Current Density

Autori
Černocká, Hana ; Izadi, Nasim ; Ostatná, Veronika ; Strmečki, Slađana

Izvornik
Electroanalysis (1040-0397) 31 (2019), 10; 2007-2011

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
BSA-polysaccharide interaction ; BSA structural transition ; Chronopotentiometric stripping ; Mercury electrode ; Peak H

Sažetak
Constant current chronopotentiometric stripping (CPS) peak H due to catalytic hydrogen evolution reaction on Hg‐containing electrodes appeared useful in the analysis of protein complexes with single‐stranded and double‐ stranded DNA as well as with peptides. In dependence on stripping current (Istr), structural transition of the protein alone or in complexes can be followed as a result of the protein exposure to electric field effects. For the first time we show here that the CPS analysis can be used for the study of the interaction of BSA with a polysaccharide namely sodium alginate (SA). BSA‐SA complex formation was accompanied by the shift of the structural transition of BSA to lower ‐Istr intensities. Another polysaccharide dextran did not alter Istr‐dependent structural transition of BSA. BSA‐SA complex can be disturbed by an electric field effect or high ionic strength confirming the electrostatic nature of BSA‐SA interaction.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti

Napomena
Institute of Biophysics ; Faculty of Science, Masaryk University

POVEZANOST RADA