Naslov
Analysis of HIV-1 diversity, primary drug resistance and transmission networks in Croatia

Autori
Oroz, Maja ; Begovac, Josip ; Planinić, Ana ; Rokić, Filip ; Lunar, Maja M. ; Zorec, Tomaž Mark ; Beluzić, Robert ; Korać, Petra ; Vugrek, Oliver ; Poljak, Mario ; Lepej, Snježana Židovec

Izvornik
Scientific Reports (2045-2322) 9 (2019), 1; 17307, 16

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
HIV, molekularna epidemiologija, transmisijski klasteri, filogenetska analiza, filodinamska analiza, sekvenciranje nove generacije
(HIV, molecular epidemiology, transmission clusters, phylogenetic analysis, phylodinamic analysis, next generation sequencing)

Sažetak
Olecular epidemiology of HIV-1 infection in treatment-naive HIV-1 infected persons from Croatia was investigated. We included 403 persons, representing 92.4% of all HIV-positive individuals entering clinical care in Croatia in 2014–2017. Overall prevalence of transmitted drug resistance (TDR) was estimated at 16.4%. Resistance to nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside RTI (NNRTIs) and protease inhibitors (PIs) was found in 11.4%, 6.7% and 2.5% of persons, respectively. Triple-class resistance was determined in 2.2% of individuals. In addition, a single case (1.0%) of resistance to integrase strand-transfer inhibitors (InSTIs) was found. Deep sequencing was performed on 48 randomly selected samples and detected additional TDR mutations in 6 cases. Phylogenetic inference showed that 347/403 sequences (86.1%) were part of transmission clusters and identified forward transmission of resistance in Croatia, even that of triple-class resistance. The largest TDR cluster of 53 persons with T215S was estimated to originate in the year 1992. Our data show a continuing need for pre-treatment HIV resistance testing in Croatia. Even though a low prevalence of resistance to InSTI was observed, surveillance of TDR to InSTI should be continued.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA