Naslov
Strontium substituted biomimetic calcium phosphate system derived from cuttlefish bone

Autori
Ressler, Antonia ; Cvetnić, Matija ; Antunović, Maja ; Marijanović, Inga ; Ivanković, Marica ; Ivanković, Hrvoje

Izvornik
Journal of biomedical materials research. Part B, Applied biomaterials (1552-4973) 108 (2020), 4; 1697-1709

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
co-substitution ; hydroxyapatite ; octacalcium phosphate ; Rietveld refinement ; strontium

Sažetak
Biomimetic triphasic strontium‐substituted calcium phosphate (CaP) powders were prepared by wet precipitation method at 50°C, using CaCO3, (NH2)2COH3PO4, and Sr(NO3)2 as reagents. Calcite was prepared from biogenic source (cuttlefish bone). The synthesized powders have been characterized by elemental analysis, Fourier transform infrared spectrometry, X‐ray diffraction, Rietveld refinement studies and cell viability test. Phase transformation and ion release were analyzed during 7 days of incubation in simulated body fluid at 37°C. The raw precipitated powders were composed of calcium deficient carbonated hydroxyapatite (HA), octacalcium phosphate (OCP), and amorphous calcium phosphate (ACP). After heat treatment at 1200°C β‐tricalcium phosphate (β‐TCP) was detected. Strontium substitution for calcium results in an increase of lattice parameters in HA, OCP, and β‐TCP. Sr2+ occupy the Ca(1) site in HA, Ca(3, 4, 7, 8) sites in OCP and Ca(1, 2, 3, 4) sites in β‐TCP. Along with Sr2+ substitution, presence of Mg2+ and Na+ ions was detected as a result of using biogenic calcium carbonate. The culture of human embryonic kidney cells indicated noncytotoxicity of the prepared CaP powders with emphasis on the cell proliferation during 3 days of culture.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kemijsko inženjerstvo, Temeljne tehničke znanosti

Napomena
Glavni autor: Antonia Ressler

POVEZANOST RADA