Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice

Kozina, Nataša; Mihaljević, Zrinka; Baus Lončar, Mirela; Mihalj, Martina; Mišir, Mihael; Radmilović, Marina; Justić, Helena; Gajović, Srećko; Šešelja, Kate; Bazina, Iva; Horvatić, Anita; Matić, Anita; Bijelić, Nikola; Rođak, Edi; Jukić, Ivana; Drenjančević, Ines

doi:10.3390/ijms20205188

Pregled bibliografske jedinice broj: 1031619

Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice

Kozina, Nataša; Mihaljević, Zrinka; Baus Lončar, Mirela; Mihalj, Martina; Mišir, Mihael; Radmilović, Marina; Justić, Helena; Gajović, Srećko; Šešelja, Kate; Bazina, Iva et al.

Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice // International journal of molecular sciences, 20 (2019), 20; 5188, 25 doi:10.3390/ijms20205188 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1031619 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice

Autori
Kozina, Nataša ; Mihaljević, Zrinka ; Baus Lončar, Mirela ; Mihalj, Martina ; Mišir, Mihael ; Radmilović, Marina ; Justić, Helena ; Gajović, Srećko ; Šešelja, Kate ; Bazina, Iva ; Horvatić, Anita ; Matić, Anita ; Bijelić, Nikola ; Rođak, Edi ; Jukić, Ivana ; Drenjančević, Ines

Izvornik
International journal of molecular sciences (1422-0067) 20 (2019), 20; 5188, 25

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Tff3 gene ; flow-induced dilation ; high salt diet ; stroke

Sažetak
High salt (HS) dietary intake leads to impaired vascular endothelium-dependent responses to various physiological stimuli, some of which are mediated by arachidonic acid (AA) metabolites. Transgenic Tff3−/− gene knockout mice (Tff3−/−/C57BL/6N) have changes in lipid metabolism which may affect vascular function and outcomes of stroke. We aimed to study the effects of one week of HS diet (4% NaCl) on vascular function and stroke induced by transient occlusion of middle cerebral artery in Tff3−/− and wild type (WT/C57BL/6N) mice. Flow-induced dilation (FID) of carotid artery was reduced in WT-HS mice, but not affected in Tff3−/−-HS mice. Nitric oxide (NO) mediated FID. NO production was decreased with HS diet. On the contrary, acetylcholine-induced dilation was significantly decreased in Tff3−/− mice on both diets and WT-HS mice. HS intake and Tff3 gene depletion affected the structural components of the vessels. Proteomic analysis revealed a significant effect of Tff3 gene deficiency on HS diet-induced changes in neuronal structural proteins and acute innate immune response proteins’ expression and Tff3 depletion, but HS diet did not increase the stroke volume, which is related to proteome modification and upregulation of genes involved mainly in cellular antioxidative defense. In conclusion, Tff3 depletion seems to partially impair vascular function and worsen the outcomes of stroke, which is moderately affected by HS diet.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA

Projekti:
HRZZ-IP-2014-09-6380 - Poremećena vazorelaksacija i endotelno-leukocitna interakcija (ELI) u razvoju aterosklerotskih lezija (V-ELI Athero) (Drenjančević, Ines, HRZZ - 2014-09) ( CroRIS)

Ustanove:
Veterinarski fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek

Profili:

Mirela Baus Lončar (autor)