Naslov
Assessment of Listerine Cool Mint mouthwash influence on possible DNA damage measured by buccal micronucleus cytome assay-preliminary results

Autori
Milić, Mirta ; Bolanča, Ivana ; Gjirlić, Dora ; Benković, Vesna

Izvornik
Genetics & Applications (2566-2937) 3 (2019), 1; 24-35

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
buccal cells ; Listerine mouthwash ; buccal micronucleus cytome assay ; genome damage

Sažetak
Listerine is a brand of mouthwash most used worldwide in oral hygene maintainance. Due to its antimicrobic and antifungal characteristics, it can stop/diminish the development of plaque and gingivitis. Among different types of this mouthwash, all 5 ingredients of Listerine Cool Mint, 21.6% ethanol and 4 herbal extracts-thymol, menthol, eucalyptol and methyl salicylate, have shown capacity to cause cell damage and buccal epithelial cells are in direct contact. Buccal micronucleus cytome assay (BMN) measures changes in differentiation as the frequency of basal/differentiated, binuclear, and cells in different phases of cell death-apoptosis/necrosis (cells with condensed chromatin, karriorhectic, pycnotic and karyolitic cells) and changes in genomic stability measured as micronuclei or nuclear buds/broken eggs frequency. Samples from 10 healthy individuals using Listerine Cool Mint mouthwash twice/day during two- weeks treatment were analyzed before and after the treatment. There was no significant influence on cell differentiation and genomic stability on the group level, although micronuclei frequency (MN) of entire group was higher after the treatment (1 vs. 1.5). We also found interindividual differences and showed that hard liquor consumers had higher MN frequency. Future studies should include more individuals, especially those that regularly consume alcohol for the analysis of possible synergistic influence and consequential increase inrisk of changes in genomic tability. Genetic polymorphisms in enzymes responsible for metabolism of ethanol should also be considered, since they may drastically influence the duration of ethanol exposure and its metabolite acetaldehyde and also influence genomic instability and possible development of oral squamous cells cancer

Izvorni jezik
Engleski

POVEZANOST RADA