Naslov
THE INFLUENCE OF A THREE-WEEK HYPOCALORIC DIET ON DNA DAMAGE PARAMETERS MEASURED BY ALKALINE COMET ASSAY AND CYTOCHALASIN B-BLOCKED MICRONUCLEUS ASSAY IN OBESE PATIENTS FROM THE SPECIAL HOSPITAL FOR EXTENDED TREATMENT OF DUGA RESA, CROATIA –PRELIMINARY RESULTS

Autori
Ožvald, Ivan ; Božičević, Dragan ; Vinković Vrček, Ivana ; Pavičić, Ivan ; Domijan, Ana Marija ; Milić, Mirta

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Genetics&Applications, Volume 2, No.2, Special edition, Book of abstracts, 1st Congress of Geneticists in Bosnia and Herzegovina with International Participation, / Bajrović, Kasim - Sarajevo : Genetics&Applications, Volume 2, No.2, Special edition, Book of abstracts, Institute for Genetic Engineering and Biotechnology, University of Sarajevo, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina, www.ingeb.unsa.ba, 2019, 31-31

Skup
1st Congress of Geneticists in Bosnia and Herzegovina with International Participation

Mjesto i datum
Sarajevo, Bosna i Hercegovina, 02.10.2019. - 04.10.2019

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
3 week calorie restriction diet ; Comet assay, Micronucleus assay, lymphocytes, DNA damage, Obesity

Sažetak
Obese subjects consistently demonstrate increased DNA damage compared to healthy individuals. Besides excess body weight, obesity is associated with comorbidities such as cardiovascular disease, type 2 diabetes mellitus, hypertension and non-alcoholic fatty liver disease, all of which are connected with oxidative stress. Increased oxidative stress could damage cellular macromolecules including DNA, with genomic instability as one of the driving forces for carcinogenesis. Although conservative weight loss regimens demonstrated beneficiary influence on DNA damage reduction, little is known whether a similar effect can be achieved with a more extensive weight loss using more restrictive calorie reduction diets, so the predictions for human population is mostly based on in vitroand animal model studies. The aim of this preliminary study was to examine the impact of a 3-week calorie reduction diet of only 500 kcal in persons with a body mass index of ≥ 35 kg/m2, without any known comorbidity and therapy, on genomic stability using cytochalasin B-blocked micronucleus assay, alkaline comet assay and modified alkaline comet assay for oxidative DNA damage measurement. Fresh whole blood samples were taken before and after the three weeks hospital treatment with the strict diet regime. The results of this preliminary study on 10 volunteers demonstrated reduced volunteers body weight, reduced levels of DNA damage in alkaline comet assay and in oxidative DNA damage. There was a slight reduction in frequency of micronuclei (parameter of whole chromosome loss or partial chromosome loss), while the frequency of nuclear buds (parameter of gene amplification) and nucleoplasmic bridges (parameter of dicentric chromosomes) remained the same. There was also an increase in the proliferation index and reduction in number of apoptosis. The results indicated a beneficiary effect of this strict calorie reduction diet and should be repeated on a higher number of participants.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita, Farmacija

Napomena
Rad je rađen na Institutu za medicinska istraživanja i medicinu rada te u Specijalnoj bolnici za produženo liječenje Duga Resa

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