Naslov
From bio-waste to bone substitute: Biomimetic chitosan/hydroxyapatite scaffolds

Autori
Antonia Ressler, Ana Gudelj, Karla Zadro, Maja Antunović, Matija Cvetnić, Marica Ivankovića, Hrvoje Ivanković

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, ostalo

Skup
The Workshop for Young Researchers in Ceramics and Glasses for Medical Applications

Mjesto i datum
Madrid, Španjolska, 10.10.2019. - 11.10.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
hydroxyapatite, chitosan, biogenic source, trace element
(hidroksiapatit, kitozan, biogen izvor, elementi u tragovima)

Sažetak
The aim of the present work was comparative study of physiochemical and biological properties of obtained chitosan/hydroxyapatite scaffolds, where hydoxyapatie was obtained from three different biogenic sources. As a source of calcium ions biogenic calcium oxide, derived from hen eggshells, sea shells and cuttlefish bone was used. The synthesized powders have been characterized by elemental analysis, Fourier transform infrared spectrometry, X-ray diffraction, and Rietveld refinement studies. Highly porous chitosan/hydroxyapatite composite structures have been prepared by freeze-gelation technique, while morphology of scaffolds was imaged by scanning electron microscopy (SEM). The as-prepared powders were composed of calcium deficient hydroxyapatite and amorphous calcium phosphate. Heat treated powders were composed of hydroxyapatie, α-tricalcium phosphate and β-tricalcium phosphate. In as-prepared powders Sr2+, Mg2+ and Na+ ions were detected as result of using biogenic precursor of Ca2+ ions. SEM observations of cross section and surface area of prepared hydroxyapatite/chitosan scaffolds have shown highly porous structure with very well interconnected pores and homogeneously dispersed hydroyapatite particles. The MTT assay of scaffolds with hydroxyapatite obtained from different biogenic sources has shown no toxicity and the live dead staining has confirmed good viability, as well as proliferation of seeded cells by the culture time.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kemijsko inženjerstvo, Temeljne tehničke znanosti

POVEZANOST RADA