Naslov
INFLUENCE OF FASTING AND REFEEDING ON GLUCOSE- 6-PHOSPHATE DEHYDROGENASE, GLUTATHIONE AND LIPID PEROXIDE CONCENTRATION IN CHICKEN BLOOD

Autori
Orban, Morana ; Stojević, Zvonko ; Vranković, Lana ; Aladrović, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstract 8th International Congress "Veterinary Science and Profession" / Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2019, 131-131

Skup
8. međunarodni kongres Veterinarska znanost i struka

Mjesto i datum
Zagreb, Hrvatska, 10.10.2019. - 12.10.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
glucose-6-phosphate dehydrogenase, glutathione concentration, lipid peroxide concentration, chicken blooD
(glucose-6-phosphate dehydrogenase, glutathione concentration, lipid peroxide concentration, chicken bloo)

Sažetak
Avian metabolism is more intense than metabolic rate in mammals. Therefore, the consumption of oxygen in avian species is more intensive, which means that birds are exposed to a higher oxidative stress due to an increased level of free radicals. The objective of this study was to determine the influence of fasting and refeeding on the concentrations of glutathione (GSH) and lipid peroxide measured as reactive products of the thiobarbituric acid (TBARS) in blood and the glucose-6-phosphate dehydrogenase (G6PDH) activity in chicken erythrocytes. At the age of 61 days, Lohman Brown cockerels were exposed to a 48-h fasting and afterwards to a 24-h refeeding. The blood for the analysis was taken from the wing vein before fasting (control values), after the 48-h fasting, and then 24 h after refeeding. The concentrations of GSH and TBARS and the activity of G6PDH were determined and expressed per gram haemoglobin. In cockerels, the concentration of GSH in blood after refeeding was significantly higher than the control and fasting values. The TBARS concentration did not show significant differences throughout the experiment. The activity of G6PDH after the 24-h refeeding significantly decreased compared to the control level. Based on the results, we can conclude that the GSH concentration during 48 h was maintained at similar levels as the control values, probably due to the transport from the liver and other organs. After refeeding, GSH was increased considering the absorption from the intestine and synthesis, which compensated the reduced G6PDH activity. Lipid peroxidation decreased as a result of a lower intensity of metabolism during fasting

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA