Naslov
The guanidiniocarbonylpyrrole - fluorophore conjugates as theragnostic tools for DPP III monitoring and inhibition

Autori
Ćehić, Mirsada ; Suć Sajko, Josipa ; Karačić, Zrinka ; Piotrowski, Patryciusz ; Šmidlehner, Tamara ; Jerić, Ivanka ; Schmuck, Carsten ; Piantanida, Ivo ; Tomić, Sanja

Izvornik
Journal of biomolecular structure & dynamics (0739-1102) 38 (2020), 13; 3790-3800

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DPP III inhibitor ; fluorescence ; pyrene ; cyanine ; guanidiniocarbonyl-pyrrole

Sažetak
Study of seven new guanidiniocarbonylpyrrole (GCP) - fluorophore conjugates interactions with dipeptidyl peptidase III (DPP III) showed that all compounds bind strongly (Ks ≈ µM) to enzyme active site, but with very different fluorimetric response (varying from quenching to strong increase), dependent on the fluorophore type and intramolecular pre-organisation of molecule. Positively charged lysine side chain improved significantly compound solubility but diminished fluorescence increase upon DPP III binding and completely abolished inhibitory effect on DPP III activity, whereas linker-neutral analogues showed stronger emission increase and were efficient enzyme inhibitors. By far the best fluorimetric response and inhibitive properties showed cyanine – GCP analogue, thus being promising lead compound for both, enzyme sensing and bio-activity inhibiting (theragnostic) studies of DPP III in the future.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA