Pregled bibliografske jedinice broj: 1026885
Analysis of IGF1 gene polymorphism and differences in litter size after first parity in Romanov sheep: preliminary results
Analysis of IGF1 gene polymorphism and differences in litter size after first parity in Romanov sheep: preliminary results // Book of abstracts of 8th international congress "Veterinary science and profession" / Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran (ur.).
Zagreb: Veterinarski fakultet Sveučilišta u Zagrebu, 2019. str. 69-69 (predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1026885 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Analysis of IGF1 gene polymorphism and differences in litter size after first parity in Romanov sheep: preliminary results
Autori
Vlahek, Ivan ; Ekert Kabalin, Anamaria ; Menčik, Sven ; Maurić, Maja ; Piplica, Aneta ; Sušić, Velimir
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of abstracts of 8th international congress "Veterinary science and profession"
/ Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2019, 69-69
Skup
8. međunarodni kongres Veterinarska znanost i struka
Mjesto i datum
Zagreb, Hrvatska, 10.10.2019. - 12.10.2019
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
IGF1 gene, polymorphism, litter size
Sažetak
Insulin-like growth factor 1 gene (IGF1 gene) controls the synthesis of insulin-like growth factor 1 (IGF1) which is involved in many physiological processes including reproduction. 5' flanking region of the ovine IGF1 gene is proven to be polymorphic, and the polymorphisms are associated with reproductive traits in some sheep breeds. The objectives of this study were to analyse ewes of high prolific breed with regard to their IGF1 genotype and litter size after first lambing. DNA was extracted from jugular vein blood of 22 Romanov ewes. Amplified PCR products of 5' flanking region of the ovine IGF1 gene were digested with HaeII restriction enzyme and allele and genotype frequencies were determined. Fischer-Freeman-Halton's test was used to test the differences in the number of ewes with specific birth type between observed genotypes. Determined level of significance was P<0.05. Digestion of PCR products with HaeII revealed three genotypes: AA, AB and BB. Allele A had a higher frequency (0.64) than allele B (0.36), and absolute genotype frequencies were as follows: AA (8), AB (12) and BB (2). Median, minimum and maximum for litter size after first parity for AA and AB genotyped ewes was 2 (1- 3), while within BB genotype one ewe had one lamb, and other two lambs. Multiple births (two or three lambs per litter) were observed in 87.5%, 75% and 50% of ewes within AA, AB and BB genotypes, respectively, and ewes with AA genotype had 12.5% and 25% more multiple births than AB and BB genotyped ewes, respectively. Observed differences were not significant (P>0.05). This preliminary study confirmed the presence of a polymorphism at 5' flanking region of the IGF1 gene in Romanov sheep. Absence of significant differences in birth type variation between genotypes may be due to the small number of animals involved, so further investigation with a larger number of sheep is recommended.
Izvorni jezik
Engleski
POVEZANOST RADA
Profili:
Aneta Piplica
(autor)
Anamaria Ekert Kabalin
(autor)
Velimir Sušić
(autor)
Maja Maurić Maljković
(autor)
Sven Menčik
(autor)
Ivan Vlahek
(autor)