Naslov
Effect of autogenous vaccine on the phylogenetic relationship and virulence profiles of avian pathogenic E. coli isolated from broiler breeder flocks

Autori
Lozica, Liča ; Horvatek Tomić, Danijela ; Repar, Jelena ; Gottstein, Željko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
8th International Congress "Veterinary Science and Profession"- Book of Abstracts / Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2019, 68-68

Skup
8. međunarodni kongres Veterinarska znanost i struka

Mjesto i datum
Zagreb, Hrvatska, 10.10.2019. - 12.10.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Escherichia coli ; APEC ; poultry ; autogenous vaccine ; phylogeny

Sažetak
Avian pathogenic E. coli (APEC) is one of the most common microbial causes of decreased production and high mortality rates in poultry farming. Poultry health protection is based on immunoprophylaxis and enables high production rates. We conducted a longitudinal study of two broiler breeder flocks on the same farm that had severe problems with colibacillosis. Since the commercial vaccine was not effective, the use of an autogenous vaccine was initiated. Flock A was vaccinated with the commercial vaccine, while Flock B was vaccinated with the autogenous vaccine. The aim of this study was to investigate the effect of the autogenous vaccine on the phylogenetic relationship and virulence profiles of APEC isolates in these two flocks. Liver and peritoneum swabs were taken from 10 and 25 hens, respectively, from the Flock A and Flock B. Thirteen APEC isolates from each flock were randomly selected for whole-genome sequencing. DNA was isolated by using the Maxwell RSC Cultured Cells DNA Kit (Promega), followed by paired-end sequencing (MiSeq, Illumina). The CGE tools Assembler, MLST and MyDbFinder were used for de novo assembly of the genomes, the detection of housekeeping and virulence genes of our interest, respectively. The concatenations made from housekeeping genes were used for the construction of a phylogenetic tree, which was done by using the Maximum Likelihood method and Kimura 2-parameter model using MEGA X. The phylogenetic tree showed the isolates clustering into multiple subgroups, mostly showing a clear distinction between the isolates originating from the Flock A and Flock B. The isolates from both flocks clustered in a separate, distant subgroup, which may be linked to a greater severity of symptoms. The virulence profiles showed a diverse frequency of certain virulence genes with a clear distinction between the flocks. The results indicate that an autogenous vaccine could affect the phylogenetic relation and virulence profiles of APEC isolates. In order to confirm the hypotheses, an extensive study should be conducted with more isolates representing the same flocks, as well as other flocks from the same farm.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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