Naslov
Influence of biosecurity measures and management practice on genetic diversity of Gallibacterium anatis isolated on poultry farms

Autori
Lozica, Liča ; Gottstein, Željko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th International Congress "Veterinary Science and Profession"- Book of Abstracts / Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2019, 67-67

Skup
8. međunarodni kongres Veterinarska znanost i struka

Mjesto i datum
Zagreb, Hrvatska, 10.10.2019. - 12.10.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Gallibacterium anatis, rpoB, phylogeny, poultry, biosecurity

Sažetak
Gallibacterium anatis is considered one of the most common bacterial causative agents of various disorders in poultry. Although it is a part of normal microflora in the upper respiratory and lower reproductive tract, it is an opportunistic pathogen and can cause serious losses in poultry production. Due to its impact on production and high prevalence on poultry farms, we wanted to compare the isolates from two farms with different rearing and management practices. The comparison was done based on the sequences of the gene encoding the ß-subunit of the RNA polymerase (rpoB), which is highly conserved within the family of Pasteurellaceae and has high discriminatory power. Bacterial strains from a broiler breeder farm (Farm 1) and a layer farm (Farm 2) were randomly selected. From those samples, DNA was extracted by using Chelex 100 (BioRad, France). The samples were amplified and visualised through polymerase chain reaction and 1% agarose gel electrophoresis, respectively. The purification of the amplicons from gel slices was done by using the ReliaPrep™ DNA Clean-up Concentration System (Promega, USA)and afterwards, the isolates were sequenced. The sequences were edited by using the BioEdit Sequence Alignment Editor and afterwards the Basic Local Alignment Search Tool (BLAST) was used to search for identical sequences available at GenBank. A phylogenetic tree was constructed by using the Maximum Likelihood method and Tamura 3-parameter model using the Molecular Evolutionary Genetics Analysis (MEGA X). The results show a high level of homogeneity of the isolates from the Farm 1, as opposed to the heterogeneity of the isolates from the Farm 2. The Farm 1 has its own rearing flocks that are reared in the conditions of higher biosecurity standards, contrary to the Farm 2 that imports pullets from multiple rearing farms and has low biosecurity standards. The G. anatis strains are frequently farm-specific, but these results indicate that low biosecurity measures and a bad management practice could greatly affect their diversity.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA