Naslov
In vivo expression profiles of dipeptidyl peptidases 8 and 9 in a mouse model of Crohn's disease

Autori
Buljevic, Suncica ; Pernjak Pugel, Ester ; Varljen, Jadranka ; Baticic, Lara ; Barisic, Karmela ; Detel, Dijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

ISBN
978-953-95551-7-5

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology "Crossroads in Life Sciences" (HDBMB2019)

Mjesto i datum
Lovran, Hrvatska, 25.09.2019. - 28.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Recenziran

Ključne riječi
DP8, DP9, CD26, Crohn's disease

Sažetak
Crohn's disease represents a chronic relapsing immunologically mediated inflammatory disorder of the gastrointestinal tract occurring when luminal antigens gain access to the underlying mucosal tissue triggering a wide range of biochemical mechanisms which are partly mediated by the enzymes of the CD26 gene family. Dipeptidyl peptidase (DP) 8 and DP9, homologs of the chief family member DPP IV/CD26, are proteases with diverse cellular functions implicated in cell interactions, apoptosis, and immune response. Their exact role in the pathogenesis of chronic autoimmune diseases, as well as the question of their presumably intracellular localization, remains to be clarified. By qPCR and immunodetection, we assessed their expression patterns as well as quantified DP8/9 enzyme activity in distinctive phases of Crohn's disease under DPP IV/CD26 deficiency. DP8 mRNA expression changed similarly as DPP IV/CD26 in development and resolution of colitis, while colon concentration increased upon inflammation onset in CD26-/- mice. DP9 expression was not affected by colitis in CD26-/- mice, but its colon concentration was significantly higher than in wild-type mice. Surprisingly, dominantly intracellular DP8 and DP9 were found in abundance in mouse serum. DP8/9 activity was found to be decreased in the inflamed colon, whereas its contribution to the overall serum DPP IV/CD26-like activity was negligible, suggesting the importance of their extra- enzymatic functions. To summarize, Crohn's disease induction generated gene, protein and enzymatic changes of DP8 and DP9 implying their involvement in inflammation development as well as healing process, especially in terms of CD26 deficiency so their exact role as well as the question of their subcellular localization should be additionally examined.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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