Naslov
Secreted Virus - Encoded Proteins Reflect Murine Cytomegalovirus productivity in Organs

Autori
Brune, Wolfram ; Hasan, Milena ; Krych, Matthaus ; Bubić, Ivan ; Jonjić, Stipan ; Koszinowski, Ulrich H.

Izvornik
The Journal of infectious diseases (0022-1899) 184 (2001), 10; 1320-1324

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Sažetak
Mouse infection with murine cytomegalovirus (MCMV) is an established model for studying human cytomegalovirus infection. In this study, the relationship was analyzed between MCMV activity in organs of infected mice and the presence of infectious virus (viremia), viral genomes (DNAemia), or secreted virus-encoded proteins in the blood. For the latter, 2 recombinant viruses were constructed that encode for the hepatitis B virus surface antigen and the secreted alkaline phosphatase, respectively, as secreted marker proteins. The secreted markers correlated better with the infection in organs than DNAemia and viremia. The marker protein assays can serve as practical and sensitive tools for longitudinal monitoring of MCMV infection in individual mice.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA